Delta-like 1 and lateral inhibition during hair cell formation in the chicken inner ear: evidence against cis-inhibition

被引:29
作者
Chrysostomou, Elena [1 ]
Gale, Jonathan E. [1 ]
Daudet, Nicolas [1 ]
机构
[1] UCL, Ear Inst, London WC1X 8EE, England
来源
DEVELOPMENT | 2012年 / 139卷 / 20期
基金
英国生物技术与生命科学研究理事会; 英国工程与自然科学研究理事会;
关键词
Lateral inhibition; Inner ear; Delta-like; 1; Notch; Pattern formation; Cis-inhibition; NOTCH SIGNALING PATHWAY; PATTERN-FORMATION; PROSENSORY PATCHES; MAMMALIAN COCHLEA; FATE CHOICES; MIND-BOMB; DROSOPHILA; EXPRESSION; DIFFERENTIATION; ZEBRAFISH;
D O I
10.1242/dev.074476
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The formation of the salt-and-pepper mosaic of hair cells and supporting cells in the sensory epithelia of the inner ear is regulated by Notch signalling and lateral inhibition, but the dynamics of this process and precise mode of action of delta-like 1 (Dll1) in this context are unclear. Here, we transfected the chicken inner ear with a fluorescent reporter that includes elements of the mammalian Hes5 promoter to monitor Notch activity in the developing sensory patches. The Hes5 reporter was active in proliferating cells and supporting cells, and Dll1 expression was highest in prospective hair cells with low levels of Notch activity, which occasionally contacted more differentiated hair cells. To investigate Dll1 functions we used constructs in which Dll1 expression was either constitutive, regulated by the Hes5 promoter, or induced by doxycycline. In support of the standard lateral inhibition model, both continuous and Hes5-regulated expression of Dll1 promoted hair cell differentiation cell-autonomously (in cis) and inhibited hair cell formation in trans. However, some hair cells formed despite contacting Dll1-overexpressing cells, suggesting that some progenitor cells are insensitive to lateral inhibition. This is not due to the cis-inhibition of Notch activity by Dll1 itself, as induction of Dll1 did not cell-autonomously reduce the activity of the Hes5 reporter in progenitor and supporting cells. Altogether, our results show that Dll1 functions primarily in trans to regulate hair cell production but also that additional mechanisms operate downstream of lateral inhibition to eliminate patterning errors in the sensory epithelia of the inner ear.
引用
收藏
页码:3764 / 3774
页数:11
相关论文
共 56 条
[51]   Characterization of supporting cell phenotype in the avian inner ear: Implications for sensory regeneration [J].
Warchol, Mark E. .
HEARING RESEARCH, 2007, 227 (1-2) :11-18
[52]   Tet-on inducible system combined with in ovo electroporation dissects multiple roles of genes in somitogenesis of chicken embryos [J].
Watanabe, Tadayoshi ;
Saito, Daisuke ;
Tanabe, Koji ;
Suetsugu, Rinako ;
Nakaya, Yukiko ;
Nakagawa, Shinichi ;
Takahashi, Yoshiko .
DEVELOPMENTAL BIOLOGY, 2007, 305 (02) :625-636
[53]   Oscillations and patterns in spatially discrete models for developmental intercellular signalling [J].
Webb, SD ;
Owen, MR .
JOURNAL OF MATHEMATICAL BIOLOGY, 2004, 48 (04) :444-476
[54]   In vivo cell sorting in complementary segmental domains mediated by Eph receptors and ephrins [J].
Xu, QL ;
Mellitzer, G ;
Robinson, V ;
Wilkinson, DG .
NATURE, 1999, 399 (6733) :267-271
[55]  
Zine A, 2000, DEVELOPMENT, V127, P3373
[56]   Hes1 and Hes5 activities are required for the normal development of the hair cells in the mammalian inner ear [J].
Zine, A ;
Aubert, A ;
Qiu, JP ;
Therianos, S ;
Guillemot, F ;
Kageyama, R ;
de Ribaupierre, F .
JOURNAL OF NEUROSCIENCE, 2001, 21 (13) :4712-4720