Expression and activity of mitogen activated protein kinases in human colorectal carcinoma

被引：15

作者：

Eggstein, S ^{[1
]}

Franke, M ^{[1
]}

Kutschka, I ^{[1
]}

Manthey, G ^{[1
]}

von Specht, BU ^{[1
]}

Ruf, G ^{[1
]}

Farthmann, EH ^{[1
]}

机构：

[1] Univ Freiburg, Chirurg Klin, Abt Allgemeine Chirurg Polklin, Dept Gen Surg, D-79106 Freiburg, Germany

来源：

GUT | 1999年 / 44卷 / 06期

关键词：

mitogen activated protein kinase (MAPK); Raf-1; colorectal cancer;

D O I：

10.1136/gut.44.6.834

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Background-Mitogen activated protein kinases (MAPKs) play a central role in the regulation of both cell growth and differentiation. They sere involved in signal transduction of oncogenes and growth factors. The role of MAPK in colonic carcinoma is unknown. Aims-To establish whether the expression and activity of p42/44 MAPKs are altered in colorectal tumours as compared with normal mucosa. Methods-The expression and activity of p42/p44 MAPK were investigated in 22 colorectal carcinomas, four adenomas, and the corresponding normal colorectal mucosa by the use of western blotting, immunoprecipitation, and in vitro kinase assays. Results-After immunoprecipitation with an antibody specific for p42 MAPK, we found significant inactivation of p42 MAPK in colonic carcinomas as well as in adenomas, whereas most sample pairs showed only minor differences in p42 MAPK expression, investigation of MAPK with an antibody capable of detecting both p42 and p44 MAPK showed a slight but significant decrease in p44 MAPK content in malignant tissues. With this antibody, only minor alterations in MAPK activity and no correlation with p42 MAPK activity were found. Conclusions-Inactivation of p42 MAPK could be associates with colonic carcinogenesis.

引用

页码：834 / 838

页数：5

共 24 条

[1] RAF-1 IS A POTENTIAL SUBSTRATE FOR MITOGEN-ACTIVATED PROTEIN-KINASE INVIVO [J].