Circulating levels of Locusta diuretic hormone: The effect of feeding

被引：43

作者：

Audsley, N ^{[1
]}

Goldsworthy, GJ ^{[1
]}

Coast, GM ^{[1
]}

机构：

[1] UNIV LONDON BIRKBECK COLL, DEPT BIOL, LONDON WC1E 7HX, ENGLAND

来源：

PEPTIDES | 1997年 / 18卷 / 01期

基金：

英国生物技术与生命科学研究理事会;

关键词：

CRF-related peptide; Locusta diuretic hormone; radioimmunoassay; Malpighian tubule secretion; postfeeding diuresis; Locusta migratoria;

D O I：

10.1016/S0196-9781(96)00234-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A radioimmunoassay was developed using I-125-labeled- [Tyr(0)]Locusta-DH and polyclonal antibodies raised against Locusta-DH(29-46). The assay had a detection limit of 50 pM, and displayed limited crossreactivity for other CRF-related peptides, but not for unrelated peptides. About 60% of the total immunoreactive material in locust hemolymph was attributable to Locusta-DH. The circulating level of diuretic hormone increases fivefold in fed insects, sufficient to stimulate primary urine production, and is correlated with the duration of the meal. This is consistent with the role of Locusta-DH in the control of postfeeding diuresis. (C) 1997 Elsevier Science Inc.

引用

页码：59 / 65

页数：7

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