Expression and cellular localization of Atrab28 during Arabidopsis embryogenesis

被引:16
作者
Arenas-Mena, C
Raynal, M
Borrell, A
Varoquaux, F
Cutanda, MC
Stacy, RAP
Pagès, M
Delseny, M
Culiáñez-Macià, FA
机构
[1] Univ Politecn Valencia, CSIC, Inst Biol Mol & Celular Plantas, Valencia 46022, Spain
[2] CSIC, Dept Mol Genet, Ctr Invest & Desenvolupament, ES-08034 Barcelona, Spain
[3] Univ Perpignan, CNRS, UMR 5545, Lab Physiol & Biol Mol Plantes, F-66860 Perpignan, France
[4] CALTECH, Div Biol, Pasadena, CA 91125 USA
[5] Univ Oslo, Div Gen Genet, N-0315 Oslo, Norway
关键词
Arabidopsis thaliana; embryo-specific gene expression; Lea proteins;
D O I
10.1023/A:1006219315562
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The maize abscisic acid (ABA)-responsive gene rab28 has been shown to be ABA-inducible in embryos and vegetative tissues, expression being mostly restricted to vascular elements during late embryogenesis. In the course of an expressed sequence tags (ESTs) programme, we have isolated an Arabidopsis thaliana gene, Atrab28, encoding the orthologue of maize rab28. The Atrab28 cDNA is 1090 bp long, including a poly(A)(+) stretch, and encodes a polypeptide of 262 amino acids. Atrab28 antibody against the recombinant protein recognizes a polipeptide of about 30 kDa and pI 6, in close agreement with the predicted molecular mass and pI. As for maize rab28, expression studies with Atrab28 revealed high specificity for embryo tissues, transcription being stimulated by the transcriptional activator abi3. In contrast, Atrab28 was not induced in vegetative tissues by ABA, osmotic stress or dehydration. The expression of Atrab28 mRNA and the accumulation of Atrab28 protein was largely restricted to provascular tissues of mature embryos and in the seed coat outer tegument and embryo and silique epidermis, as revealed by in situ hybridization and immunocytochemistry with anti-Atrab28 antibodies.
引用
收藏
页码:355 / 363
页数:9
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