A PCR-marker for the CMS1 inducing cytoplasm in chives derived from recombination events affecting the mitochondrial gene atp9

被引：27

作者：

Engelke, T ^{[1
]}

Tatlioglu, T ^{[1
]}

机构：

[1] Univ Hannover, Abt Angew Genet, D-30419 Hannover, Germany

来源：

THEORETICAL AND APPLIED GENETICS | 2002年 / 104卷 / 04期

关键词：

Allium schoenoprasum; cytoplasmic male sterility;

D O I：

10.1007/s00122-001-0770-7

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The complete coding and 3'-flanking region of the mitochondria) gene atp9 of chives (Allium schoenoprasum L.) was determined in order to develop primers that allow the identification of atp9-related sequences in subsequent PCR-amplifications. One of these sequences is of a chimerical nature, consisting of atp9-homologous regions on its end, interrupted by an insertion that is composed of ono atp6-homologous part and one part of unknown origin. This PCR-fragment is 762 by in size and exclusively amplified in the sterility inducing cytoplasm of CMS1. Thus it can be used as a PCR-marker in order to distinguish this cytoplasm type from the remaining cytoplasm types of chives. The chimerical marker sequence forms a putative open reading frame (orfA501), from which CMS1 might originate.

引用

页码：698 / 702

页数：5