Translational Applications of Flow Cytometry in Clinical Practice

被引：84

作者：

Jaye, David L. ^{[1
,2
]}

Bray, Robert A. ^{[1
]}

Gebel, Howard M. ^{[1
]}

Harris, Wayne A. C. ^{[2
,3
]}

Waller, Edmund K. ^{[2
,3
]}

机构：

[1] Emory Univ, Dept Pathol & Lab Med, Atlanta, GA 30322 USA

[2] Emory Univ, Winship Canc Inst, Atlanta, GA 30322 USA

[3] Emory Univ, Dept Hematol & Med Oncol, Atlanta, GA 30322 USA

来源：

JOURNAL OF IMMUNOLOGY | 2012年 / 188卷 / 10期

关键词：

HEMATOPOIETIC STEM-CELLS; MULTIPLE-MYELOMA; BONE-MARROW; CROSS-MATCH; TRANSPLANTATION; ENGRAFTMENT; TECHNOLOGY; EXPRESSION; THERAPY; DISEASE;

D O I：

10.4049/jimmunol.1290017

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Flow cytometry has evolved over the past 30 y from a niche laboratory technique to a routine tool used by clinical pathologists and immunologists for diagnosis and monitoring of patients with cancer and immune deficiencies. Identification of novel patterns of expressed Ags has led to the recognition of cancers with unique pathophysiologies and treatment strategies. FAGS had permitted the isolation of tumor-free populations of hematopoietic stem cells for cancer patients undergoing stem cell transplantation. Adaptation of flow cytometry to the analysis of multiplex arrays of fluorescent beads that selectively capture proteins and specific DNA sequences has produced highly sensitive and rapid methods for high through-put analysis of cytolcines, Abs, and HLA genotypes. Automated data analysis has contributed to the development of a "cytomics" field that integrates cellular physiology, genomics, and proteomics. In this article, we review the impact of the flow cytometer in these areas of medical practice. The Journal of Immunology, 2012, 188: 4715-4719.

引用

页码：4715 / 4719

页数：5

共 32 条

[1] CD4 immunophenotyping in HIV infection [J].

Barnett, David ;

Walker, Brooke ;

Landay, Alan ;

Denny, Thomas N. .

NATURE REVIEWS MICROBIOLOGY, 2008, 6 (11) :S7-S15

[2] Lymphoid reconstitution after autologous PBSC transplantation with FACS-sorted CD34+ hematopoietic progenitors [J].

Bomberger, C ;

Singh-Jairam, M ;

Rodey, G ;

Guerriero, A ;

Yeager, AM ;

Fleming, WH ;

Holland, HK ;

Waller, EK .

BLOOD, 1998, 91 (07) :2588-2600

[3] Clinical Cytometry and Progress in HLA Antibody Detection [J].

Bray, Robert A. ;

Tarsitani, Christine ;

Gebel, Howard M. ;

Lee, Jar-How .

RECENT ADVANCES IN CYTOMETRY, PART B: ADVANCES IN APPLICATIONS, FIFTH EDITION, 2011, 103 :285-310

[4] Calculated PRA: Initial Results Show Benefits for Sensitized Patients and a Reduction in Positive Crossmatches [J].

Cecka, J. M. ;

Kucheryavaya, A. Y. ;

Reinsmoen, N. L. ;

Leffell, M. S. .

AMERICAN JOURNAL OF TRANSPLANTATION, 2011, 11 (04) :719-724

[5]

Cesbron-Gautier A, 2004, ANN BIOL CLIN-PARIS, V62, P93

[6] High Risk of Graft Failure in Patients With Anti-HLA Antibodies Undergoing Haploidentical Stem-Cell Transplantation [J].

Ciurea, Stefan O. ;

de Lima, Marcos ;

Cano, Pedro ;

Korbling, Martin ;

Giralt, Sergio ;

Shpall, Elizabeth J. ;

Wang, Xuemei ;

Thall, Peter F. ;

Champlin, Richard E. ;

Fernandez-Vina, Marcelo .

TRANSPLANTATION, 2009, 88 (08) :1019-1024

[7]

CIVIN CI, 1984, J IMMUNOL, V133, P157

[8] Interpretation of Positive Flow Cytometric Crossmatch in the Era of the Single-Antigen Bead Assay [J].

Couzi, Lionel ;

Araujo, Caroline ;

Guidicelli, Gwendaline ;

Bachelet, Thomas ;

Moreau, Karine ;

Morel, Delphine ;

Robert, Gregoire ;

Wallerand, Herve ;

Moreau, Jean-Francois ;

Taupin, Jean-Luc ;

Merville, Pierre .

TRANSPLANTATION, 2011, 91 (05) :527-535

[9]

GAROVOY MR, 1983, TRANSPLANT P, V15, P1939

[10]

Herzenberg LA, 2002, CLIN CHEM, V48, P1819

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