Arabidopsis Brassinosteroid-overproducing gulliver3-D/dwarf4-D mutants exhibit altered responses to Jasmonic acid and pathogen

被引:46
作者
Kim, Bokyung [1 ]
Fujioka, Shozo [2 ]
Kwon, Mi [1 ]
Jeon, Jihyun [1 ]
Choe, Sunghwa [1 ,3 ,4 ]
机构
[1] Seoul Natl Univ, Sch Biol Sci, Coll Nat Sci, Seoul 151747, South Korea
[2] RIKEN Adv Sci Inst, Wako, Saitama 3510198, Japan
[3] Adv Inst Convergence Technol, Suwon 443270, Gyeonggi Do, South Korea
[4] Seoul Natl Univ, Plant Genom & Breeding Inst, Seoul 151921, South Korea
关键词
DWARF4; Brassinosteroids; Jasmonate; Salicylic acid; Stress; BIOSYNTHETIC-PATHWAY; DWARF4; EXPRESSION; GENE-EXPRESSION; AUXIN; GROWTH; CYTOCHROME-P450; ELONGATION; CORONATINE; CONVERSION; ETHYLENE;
D O I
10.1007/s00299-012-1381-2
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Arabidopsis gulliver3 - D/dwarf4 - D displays growth-promoting phenotypes due to activation tagging of a key brassinosteroid biosynthetic gene DWARF4. In gul3-D/dwf4-D , the Jasmonate and Salicylate signaling pathways were relatively activated and suppressed, respectively. Energy allocation between growth and defense is elegantly balanced to achieve optimal development in plants. Brassinosteroids (BRs), steroidal hormones essential for plant growth, are regulated by other plant hormones, including auxin and jasmonates (JA); auxin stimulates the expression of a key brassinosteroid (BR) biosynthetic gene, DWARF4 (DWF4), whereas JA represses it. To better understand the interaction mechanisms between growth and defense, we isolated a fast-growing mutant, gulliver3-D (gul3-D), that resulted from the activation tagging of DWF4, and examined the response of this mutant to defense signals, including JA, Pseudomonas syringae pv. tomato (Pst DC3000) infection, and wounding. The degree of root growth inhibition following MeJA treatment was significantly decreased in gul3-1D/dwf4-5D relative to the wild type, suggesting that JA signaling is partially desensitized in gul3-1D. Quantitative RT-PCR analysis of the genes involved in JA and salicylic acid (SA) responses, including MYC2, PDF1.2, CORI3, PR1, and PR2, revealed that JA signaling was preferentially activated in gul3-1D, whereas SA signaling was suppressed. As a result, gul3-1D was more susceptible to a biotrophic pathogen, Pst DC3000. Based on our results, we propose a model in which BR and JA cooperate to balance energy allocation between growth and defense responses. In ambient conditions, BRs promote plant growth; however, when stresses trigger JA signaling, JA compromises BR signaling by downregulating DWF4 expression.
引用
收藏
页码:1139 / 1149
页数:11
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