Selective activation of phospholipase D2 by unsaturated fatty acid

被引：81

作者：

Kim, JH

Kim, Y

Lee, SD

Lopez, I

Arnold, RS

Lambeth, JD

Suh, PG

Ryu, SH ^{[1
]}

机构：

[1] Pohang Univ Sci & Technol, Dept Life Sci, Pohang 790784, South Korea

[2] Pohang Univ Sci & Technol, Sch Environm Engn, Pohang 790784, South Korea

[3] Emory Univ, Sch Med, Dept Biochem, Atlanta, GA 30322 USA

来源：

FEBS LETTERS | 1999年 / 454卷 / 1-2期

关键词：

phospholipase D2; oleic acid; phosphatidylinositol 4,5-bisphosphate;

D O I：

10.1016/S0014-5793(99)00745-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Although oleate has been implicated in the regulation of phospholipase D (PLD) activity, the molecular identity of the oleate-stimulated PLD is still poorly understood. We now report that oleate selectively stimulates the enzymatic activity of PLD2 but not of PLD1, with an optimal concentration of 20 mu M in vitro. Intriguingly, phosphatidylinositol 4,5-bisphosphate (PIP2) synergistically stimulates the oleate-dependent PLD2 activity with an optimal concentration of 2.5 mu M. These results provide the first evidence that oleate is a PLD2-specific activating factor and PLD2 activity is synergistically stimulated by oleate and PIP2, (C) 1999 Federation of European Biochemical Societies.

引用

页码：42 / 46

页数：5

共 27 条

[1] ADP-RIBOSYLATION FACTOR, A SMALL GTP-DEPENDENT REGULATORY PROTEIN, STIMULATES PHOSPHOLIPASE-D ACTIVITY [J].