Gas chromatographic-tandem mass spectrometric quantification of human plasma and urinary nitrate after its reduction to nitrite and derivatization to the pentafluorobenzyl derivative

被引：19

作者：

Tsikas, D ^{[1
]}

Gutzki, FM ^{[1
]}

Sandmann, J ^{[1
]}

Schwedhelm, E ^{[1
]}

Frölich, JC ^{[1
]}

机构：

[1] Hannover Med Sch, Inst Clin Pharmacol, D-30623 Hannover, Germany

来源：

JOURNAL OF CHROMATOGRAPHY B | 1999年 / 731卷 / 02期

关键词：

tandem mass spectrometry; derivatization; GC; nitrate; nitrite;

D O I：

10.1016/S0378-4347(99)00244-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Gas chromatography-mass spectrometry (GC-MS) of nitrite as its pentafluorobenzyl derivative in the negative-ion chemical ionization mode is a useful analytical tool to quantify accurately and sensitively nitrite and nitrate after its reduction to nitrite in various biological fluids. In the present study we demonstrate the utility of GC-tandem MS to quantify nitrate in human plasma and urine. Our present results verify human plasma and urine levels of nitrite and nitrate measured previously by GC-MS. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：285 / 291

页数：7

共 7 条

[1] DETERMINATION OF ENDOTHELIUM-DERIVED NITRITE NITRATE BY GAS-CHROMATOGRAPHY TANDEM MASS-SPECTROMETRY USING (N-15)NANO2 AS INTERNAL STANDARD [J].

GUTZKI, FM ;

TSIKAS, D ;

ALHEID, U ;

FROLICH, JC .

BIOLOGICAL MASS SPECTROMETRY, 1992, 21 (02) :97-102

[2] THE L-ARGININE-NITRIC OXIDE PATHWAY IS THE MAJOR SOURCE OF PLASMA NITRITE IN FASTED HUMANS [J].

RHODES, PM ;

LEONE, AM ;

FRANCIS, PL ;

STRUTHERS, AD ;

MONCADA, S .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1995, 209 (02) :590-596

[3] MASS-SPECTROMETRY - CALCULATIONS [J].

SCHOELLER, DA .

JOURNAL OF CLINICAL PHARMACOLOGY, 1986, 26 (06) :396-399

[4] MICRODETERMINATION OF NITRATES AND NITRITES IN SALIVA, BLOOD, WATER, AND SUSPENDED PARTICULATES IN AIR BY GAS-CHROMATOGRAPHY [J].

TESCH, JW ;

REHG, WR ;

SIEVERS, RE .

JOURNAL OF CHROMATOGRAPHY, 1976, 126 (NOV3) :743-755

[5]

Tsikas D, 1998, J CHROMATOGR B, V715, P441

[6] Measurement of nitrite and nitrate in biological fluids by gas chromatography-mass spectrometry and by the Griess assay: Problems with the Griess assay - Solutions by gas chromatography-mass spectrometry [J].

Tsikas, D ;

Gutzki, FM ;

Rossa, S ;

Bauer, H ;

Neumann, C ;

Dockendorff, K ;

Sandmann, J ;

Frolich, JC .

ANALYTICAL BIOCHEMISTRY, 1997, 244 (02) :208-220

[7] QUANTIFICATION OF NITRITE AND NITRATE IN HUMAN URINE AND PLASMA AS PENTAFLUOROBENZYL DERIVATIVES BY GAS-CHROMATOGRAPHY MASS-SPECTROMETRY USING THEIR N-15-LABELED ANALOGS [J].

TSIKAS, D ;

BOGER, RH ;

BODEBOGER, SM ;

GUTZKI, FM ;

FROLICH, JC .

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS, 1994, 661 (02) :185-191

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