The osteogenic activity of strontium loaded titania nanotube arrays on titanium substrates

被引：280

作者：

Zhao, Lingzhou ^{[1
]}

Wang, Hairong ^{[2
]}

Huo, Kaifu ^{[2
,3
,4
]}

Zhang, Xuming ^{[3
]}

Wang, Wei ^{[5
]}

Zhang, Yumei ^{[5
]}

Wu, Zhifen ^{[1
]}

Chu, Paul K. ^{[3
]}

机构：

[1] Fourth Mil Med Univ, Sch Stomatol, Dept Periodontol & Oral Med, Xian 710032, Peoples R China

[2] Wuhan Univ Sci & Technol, Sch Mat & Met, Wuhan 430081, Peoples R China

[3] City Univ Hong Kong, Dept Phys & Mat Sci, Kowloon, Hong Kong, Peoples R China

[4] Chinese Acad Sci, Shanghai Inst Ceram, State Key Lab High Performance Ceram & Superfine, Shanghai 200050, Peoples R China

[5] Fourth Mil Med Univ, Sch Stomatol, Dept Prosthet Dent, Xian 710032, Peoples R China

来源：

BIOMATERIALS | 2013年 / 34卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Titania nanotubes; Strontium; Protein adsorption; Mesenchymal stem cells; Osteogenic differentiation; CALCIUM-SENSING RECEPTOR; MESENCHYMAL STEM-CELLS; MARROW STROMAL CELLS; IN-VITRO; OSTEOBLAST DIFFERENTIATION; HYDROXYAPATITE COATINGS; GENE-EXPRESSION; TIO2; NANOTUBES; BONE-FORMATION; RANELATE;

D O I：

10.1016/j.biomaterials.2012.09.041

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

Development of biomedical titanium implants with high osteogenic ability for fast and good osseointegration under normal as well as osteoporotic conditions is hotly pursued. Strontium (Sr) loaded nanotubular structures (NT-Sr) that allow controlled and long-term Sr release are expected to yield favorable osteogenic effects and properties. NT-Sr structures with different tube diameters are fabricated by hydrothermal treatment of titania nanotubes formed at 10 and 40 V (NT10 and NT40). The loaded Sr amounts are regulated by the hydrothermal treatment time of 1 and 3 h (samples NT10-Sr1, NT10-Sr3, NT40-Sr1 and NT40-Sr3) in the Sr(OH)(2) solution. Long lasting and controllable Sr release is observed from the NT-Sr samples with no cytotoxicity. The samples NT10 and NT10-Sr have multiple nanocues, comprising bundles of nanotubes of less than or equal to 30 nm with bundle diameters between 100 and 400 nm separated by about 80 nm. Sr incorporation enhances proliferation of rat mesenchymal stem cells (MSCs) on the NT structure, especially NT10-Sr which promotes the spread of the MSCs into a polygonal osteoblastic shape. Both the NT and NT-Sr samples promote osteogenesis to varying degrees as indicated by gene expression and among the various samples, samples NT10-Sr3 and NT40-Sr significantly up-regulate the expressions of the osteogenesis related genes in the absence of an extra osteogenic agent. Samples NT10 and NT10-Sr generate big nodular alkaline phosphatase (ALP) products and induce extracellular matrix (ECM) mineralization, and the effects on NT10-Sr3 are most obvious due to the multiple scaled nanostructure and proper amount of incorporated Sr. In comparison, less ALP products and failure to induce ECM mineralization are observed from sample NT40-Sr, possibly due to cell function impairment by the uneven protein distribution. NT10-Sr3 which shows excellent osteogenic properties is very attractive and has large clinical potential. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：19 / 29

页数：11

共 47 条

[1]

Strontium ranelate improves bone resistance by increasing bone mass and improving architecture in intact female rats [J].

Ammann, P ;

Shen, V ;

Robin, B ;

Mauras, Y ;

Bonjour, JP ;

Rizzoli, R .

JOURNAL OF BONE AND MINERAL RESEARCH, 2004, 19 (12) :2012-2020

[2]

Osteoblast adhesion on biomaterials [J].

Anselme, K .

BIOMATERIALS, 2000, 21 (07) :667-681

[3]

The use of nanoscale topography to modulate the dynamics of adhesion formation in primary osteoblasts and ERK/MAPK signalling in STRO-1+enriched skeletal stem cells [J].

Biggs, Manus J. P. ;

Richards, R. Geoff ;

Gadegaard, NikolaJ ;

Wilkinson, Chris D. W. ;

Oreffo, Richard O. C. ;

Dalby, Matthew J. .

BIOMATERIALS, 2009, 30 (28) :5094-5103

[4]

Nanotopographical modification: a regulator of cellular function through focal adhesions [J].

Biggs, Manus Jonathan Paul ;

Richards, R. Geoff ;

Dalby, Matthew J. .

NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE, 2010, 6 (05) :619-633

[5]

Dual effect of strontium ranelate:: Stimulation of osteoblast differentiation and inhibition of osteoclast formation and resorption in vitro [J].

Bonnelye, Edith ;

Chabadel, Anne ;

Saltel, Frederic ;

Jurdic, Pierre .

BONE, 2008, 42 (01) :129-138

[6]

A new insight into the dissociating effect of strontium on bone resorption and formation [J].

Braux, Julien ;

Velard, Frederic ;

Guillaume, Christine ;

Bouthors, Sylvie ;

Jallot, Edouard ;

Nedelec, Jean-Marie ;

Laurent-Maquin, Dominique ;

Laquerriere, Patrice .

ACTA BIOMATERIALIA, 2011, 7 (06) :2593-2603

[7]

Strontium-substituted hydroxyapatite coatings synthesized by pulsed-laser deposition: In vitro osteoblast and osteoclast response [J].

Capuccini, C. ;

Torricelli, P. ;

Sima, F. ;

Boanini, E. ;

Ristoscu, C. ;

Bracci, B. ;

Socol, G. ;

Fini, M. ;

Mihailescu, I. N. ;

Bigi, A. .

ACTA BIOMATERIALIA, 2008, 4 (06) :1885-1893

[8]

Strontium ranelate promotes osteoblastic differentiation and mineralization of murine bone marrow stromal cells: Involvement of prostaglandins [J].

Choudhary, Shilpa ;

Halbout, Philippe ;

Alander, Cynthia ;

Raisz, Lawrence ;

Pilbeam, Carol .

JOURNAL OF BONE AND MINERAL RESEARCH, 2007, 22 (07) :1002-1010

[9]

Systematic strontium substitution in hydroxyapatite coatings on titanium via micro-arc treatment and their osteoblast/osteoclast responses [J].

Chung, Chi-Jen ;

Long, Han-Yun .

ACTA BIOMATERIALIA, 2011, 7 (11) :4081-4087

[10]

In vitro effects of strontium ranelate on the extracellular calcium-sensing receptor [J].

Coulombe, J ;

Faure, H ;

Robin, B ;

Ruat, M .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2004, 323 (04) :1184-1190

← 1 2 3 4 5 →