Biosynthesis of terpenoids:: 4-Diphosphocytidyl-2C-methyl-D-erythritol synthase of Arabidopsis thaliana

被引：89

作者：

Rohdich, F

Wungsintaweekul, J

Eisenreich, W

Richter, G

Schuhr, CA

Hecht, S

Zenk, MH

Bacher, A

机构：

[1] Tech Univ Munich, Lehrstuhl Organ Chem & Biochem, D-85747 Garching, Germany

[2] Univ Halle, Biozentrum Pharm, D-06120 Halle, Germany

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2000年 / 97卷 / 12期

关键词：

D O I：

10.1073/pnas.97.12.6451

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A hypothetical gene with similarity to the ispD gene of Escherichia coli was cloned from Arabidopsis thaliana cDNA. The ORF of 909 bp specifies a protein of 302 amino acid residues. The cognate chromosomal gene consists of 2,071 bp and comprises 11 introns with a size range of 78-202 bp. A fragment comprising amino acid residues 76-302 was expressed in a recombinant E. coli strain. The protein was purified to homogeneity and was shown to catalyze the formation of 4-diphosphocytidyl-2C-methyl-D-erythritol from 2C-methyl-D-erythritol 4-phosphate with a specific activity of 67 mu mol.min(-1) mg(-1). The Michaelis constants for 4-diphosphocytidyl-2C-methyl-D-erythritol and CTP were 500 mu M and 114 mu M, respectively.

引用

收藏

页码：6451 / 6456

页数：6

相关论文

共 27 条

[1] Terpenoid biosynthesis from 1-deoxy-D-xylulose in higher plants by intramolecular skeletal rearrangement [J].

Arigoni, D ;

Sagner, S ;

Latzel, C ;

Eisenreich, W ;

Bacher, A ;

Zenk, MH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (20) :10600-10605

[2] Dedicated roles of plastid transketolases during the early onset of isoprenoid biogenesis in pepper fruits [J].

Bouvier, F ;

d'Harlingue, A ;

Suire, C ;

Backhaus, RA ;

Camara, B .

PLANT PHYSIOLOGY, 1998, 117 (04) :1423-1431

[3]

BROERS STJ, 1994, THESIS EIDEGENOSSICH

[4]

BULLOCK WO, 1987, BIOTECHNIQUES, V5, P376

[5] The deoxyxylulose phosphate pathway of terpenoid biosynthesis in plants and microorganisms [J].

Eisenreich, W ;

Schwarz, M ;

Cartayrade, A ;

Arigoni, D ;

Zenk, MH ;

Bacher, A .

CHEMISTRY & BIOLOGY, 1998, 5 (09) :R221-R233

[6] ChloroP, a neural network-based method for predicting chloroplast transit peptides and their cleavage sites [J].

Emanuelsson, O ;

Nielsen, H ;

Von Heijne, G .

PROTEIN SCIENCE, 1999, 8 (05) :978-984

[7] Biosynthesis of terpenoids:: YgbB protein converts 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate to 2C-methyl-D-erythritol 2,4-cyclodiphosphate [J].

Herz, S ;

Wungsintaweekul, J ;

Schuhr, CA ;

Hecht, S ;

Lüttgen, H ;

Sagner, S ;

Fellermeier, M ;

Eisenreich, W ;

Zenk, MH ;

Bacher, A ;

Rohdich, F .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (06) :2486-2490

[8] Inhibitors of the nonmevalonate pathway of isoprenoid biosynthesis as antimalarial drugs [J].

Jomaa, H ;

Wiesner, J ;

Sanderbrand, S ;

Altincicek, B ;

Weidemeyer, C ;

Hintz, M ;

Türbachova, I ;

Eberl, M ;

Zeidler, J ;

Lichtenthaler, HK ;

Soldati, D ;

Beck, E .

SCIENCE, 1999, 285 (5433) :1573-1576

[9] An efficient preparation of 2-C-methyl-D-erythritol 4-phosphoric acid and its derivatives [J].

Kis, K ;

Wungsintaweekul, J ;

Eisenreich, W ;

Zenk, MH ;

Bacher, A .

JOURNAL OF ORGANIC CHEMISTRY, 2000, 65 (02) :587-592

[10] Formation of 4-(cytidine 5′-diphospho)-2-C-methyl-D-erythritol from 2-C-methyl-D-erythritol 4-phosphate by 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, a new enzyme in the nonmevalonate pathway [J].

Kuzuyama, T ;

Takagi, M ;

Kaneda, K ;

Dairi, T ;

Seto, H .

TETRAHEDRON LETTERS, 2000, 41 (05) :703-706