Epidermal growth factor and human growth hormone accelerate adaptation after massive enterectomy in an additive, nutrient-dependent, and site-specific fashion

被引:42
作者
Iannoli, P
Miller, JH
Ryan, CK
Gu, LH
Ziegler, TR
Sax, HC
机构
[1] UNIV ROCHESTER,MED CTR,DEPT SURG & PATHOL,ROCHESTER,NY 14642
[2] EMORY UNIV,SCH MED,DEPT MED,DIV ENDOCRINOL & METAB,ATLANTA,GA
关键词
D O I
10.1016/S0039-6060(97)90079-9
中图分类号
R61 [外科手术学];
学科分类号
摘要
Background. After massive enterectomy (ME), remnant intestine undergoes compensatory adaptation. Epidermal growth factor (EGF) and human growth hormone (hGH) have each been shown to enhance total length small intestine nutrient transport after ME. This study aims to determine the differential effects of EGF and hGH on proximal and distal small intestinal remnants after ME. Methods. New Zealand white rabbits underwent 70% mid-jejunoileal resection. After 1 week, animals received hGH (0.2 mg/kg/day), EGF (1.5 mu g/kg/hr), hGH + EGF, or vehicle (equal volume) for 7 days. Sodium-dependent uptake of glucose, glutamine, alanine, leucine, and arginine into brush border membrane vesicles was quantitated Serum insulin-like growth factor-1 concentrations as well as proximal and distal villus and microvillus heights were measured. IGF binding protein-3 and -4 mRNA expression was determined in full-thickness proximal and distal gut remnants. Results. Concomitant hGH and EGF treatment up-regulates glucose (100%), glutamine (80%), and leucine (60%) transport in the proximal remnant; alanine (150%) and arginine (400%) transport in the distal remnant; and microvillus height (25% to 35%) both proximally and distally. Serum IGF-I levels and gross villus heights were not different among groups. Conclusions. Co-infusion of hGH and EGF accelerates intestinal adaptation after ME in an additive, nutrient-dependent, and site-specific fashion via enhanced nutrient transport as well as microvillus hypertrophy.
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页码:721 / 728
页数:8
相关论文
共 24 条
  • [11] RECEPTORS FOR INSULIN-LIKE GROWTH FACTOR-I AND FACTOR-II IN RAT GASTROINTESTINAL EPITHELIUM
    LABURTHE, M
    ROUYERFESSARD, C
    GAMMELTOFT, S
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY, 1988, 254 (03): : G457 - G462
  • [12] LIVER TEST ALTERATIONS WITH TOTAL PARENTERAL-NUTRITION AND NUTRITIONAL-STATUS
    LEASEBURGE, LA
    WINN, NJ
    SCHLOERB, PR
    [J]. JOURNAL OF PARENTERAL AND ENTERAL NUTRITION, 1992, 16 (04) : 348 - 352
  • [13] Mantell Mark P., 1993, Surgical Forum, V44, P1
  • [14] SHORT BOWEL SYNDROME
    NIGHTINGALE, JMD
    WALKER, ER
    BURNHAM, WR
    FARTHING, MJG
    LENNARDJONES, JE
    [J]. DIGESTION, 1990, 45 : 77 - 83
  • [15] SALLOUM RM, 1993, SURGERY, V113, P552
  • [16] SCHEVING LA, 1989, J BIOL CHEM, V264, P1735
  • [17] Octreotide diminishes luminal nutrient transport activity, which is reversed by epidermal growth factor
    Seydel, AS
    Miller, JH
    Sarac, TP
    Ryan, CK
    Chey, WY
    Sax, HC
    [J]. AMERICAN JOURNAL OF SURGERY, 1996, 172 (03) : 267 - 271
  • [18] STENLING R, 1981, CELL TISSUE RES, V217, P11
  • [19] AGGRESSIVE NUTRITIONAL SUPPORT DOES NOT PREVENT PROTEIN LOSS DESPITE FAT GAIN IN SEPTIC INTENSIVE-CARE PATIENTS
    STREAT, SJ
    BEDDOE, AH
    HILL, GL
    [J]. JOURNAL OF TRAUMA-INJURY INFECTION AND CRITICAL CARE, 1987, 27 (03) : 262 - 266
  • [20] SPECIFIC RECEPTORS FOR EPIDERMAL GROWTH-FACTOR IN RAT INTESTINAL MICROVILLUS MEMBRANES
    THOMPSON, JF
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY, 1988, 254 (03): : G429 - G435