Synthesis of magnetic microspheres with immobilized metal ions for enrichment and direct determination of phosphopeptides by matrix-assisted laser desorption ionization mass spectrometry

被引：358

作者：

Xu, Xiuqing

Deng, Chunhui ^{[1
]}

Gao, Mingxia

Yu, Wenjia

Yang, Pengyuan

Zhang, Xiangmin

机构：

[1] Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China

[2] Fudan Univ, Ctr Proteom Res, Shanghai 200433, Peoples R China

来源：

ADVANCED MATERIALS | 2006年 / 18卷 / 24期

关键词：

D O I：

10.1002/adma.200601546

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Core/shell structured magnetic silica microspheres with immobilized Fe3+ (see image) are prepared with a simple method. Using a magnetic field, the microspheres are quickly, efficiently, and specifically enriched. Phosphopeptide identification can then be achieved through mass spectroscopy. This is a new method for the enrichment of phosphopeptides and opens up the possibility of other new applications using the microspheres.

引用

收藏

页码：3289 / +

页数：6

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