Mapping of repeated DNA sequences in plant chromosomes by PRINS and C-PRINS

被引：48

作者：

Kubalakova, M

Macas, J

Dolezel, J

机构：

[1] DE MONTFORT UNIV,NORMAN BORLAUG CTR PLANT SCI RES,INST EXPT BOT,CZ-77200 OLOMOUC,CZECH REPUBLIC

[2] INST PLANT MOL BIOL,CZ-37005 CESKE BUDEJOVICE,CZECH REPUBLIC

来源：

THEORETICAL AND APPLIED GENETICS | 1997年 / 94卷 / 6-7期

关键词：

PRINS; C-PRINS; ribosomal RNA genes; telomeres; FokI sequence; repetitive DNA; plant chromosomes;

D O I：

10.1007/s001220050475

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The primed in situ DNA labelling (PRINS) procedure was optimised for the rapid physical mapping of several types of repetitive DNA sequences on the mitotic chromosomes of Vicia faba, Pisum sativum and Secale cereale. A localization of the highly repeated FokI sequence on V. faba chromosomes was achieved after a 7-min total reaction time. In addition, we report a procedure for direct cycling-PRINS (C-PRINS), a variation of PRINS which involves a sequence of thermal cycles analogous to the polymerase chain reaction. Compared to PRINS, C-PRINS was more sensitive. Further work is needed to improve the sensitivity of the reaction to allow for the reliable detection of low-copy DNA sequences.

引用

页码：758 / 763

页数：6

共 41 条

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