Rhesus offspring produced by intracytoplasmic injection of testicular sperm and elongated spermatids

被引:29
作者
Hewitson, L
Martinovich, C
Simerly, C
Takahashi, D
Schatten, G
机构
[1] Oregon Hlth Sci Univ, Oregon Reg Primate Res Ctr, Div Reprod Sci, Beaverton, OR USA
[2] Oregon Hlth Sci Univ, Oregon Reg Primate Res Ctr, Dept Obstet & Gynecol, Beaverton, OR USA
[3] Univ Pittsburgh, Pittsburgh Dev Ctr, Magee Womens Res Inst, Pittsburgh, PA USA
关键词
assisted reproduction; non-human primates; animal models; male infertility; spermatids; fertilization;
D O I
10.1016/S0015-0282(01)03281-2
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Objective: To establish pregnancies in rhesus monkeys using testicular sperm and elongated spermatids injected into oocytes. Design: Comparative animal study. Setting: Regional Primate Research Center. Animal(s): Prime, fertile rhesus monkeys. Intervention(s): Oocytes collected by laparoscopy from gonadotropin-stimulated female rhesus monkeys were injected with testicular sperm or elongated spermatids obtained from the testis of males. Cleavage stage embryos were transferred to surrogate females. Main Outcome Measure(s): Fertilization, ernbryo cleavage, and the establishment of pregnancies. Fertilization failures were fixed and processed for the detection or microtubules and chromatin configurations. Result(s): Fertilization, assessed by the presence of two pronuclei within 15 hours after injection, was 60% for intracytoplasmic sperm injection with testicular sperm and 47% for elongated spermatid injection. Fertilized zygotes co-cultured in Connaughts Medical Research Labs (CMRL) medium on a Buffalo Rat Liver cell monolayer resulted in hatched blastocysts after testicular sperm extraction-intracytoplasmic sperm injection and elongated spermatids. Embryos transferred at the 4- to 8-cell stage gave rise to three pregnancies: 2/3 from testicular sperm and 1/1 from an elongated spermatid. Three healthy infants were delivered by cesarean. Oocytes that failed to fertilize typically remained arrested in metaphase of meiosis. Conclusion(s): Testicular sperm and elongated spermatids can be used fur fertilization in the rhesus monkey resulting in live births. (C) 2002 by American Society for Reproductive Medicine.
引用
收藏
页码:794 / 801
页数:8
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