Rhesus offspring produced by intracytoplasmic injection of testicular sperm and elongated spermatids

被引:29
作者
Hewitson, L
Martinovich, C
Simerly, C
Takahashi, D
Schatten, G
机构
[1] Oregon Hlth Sci Univ, Oregon Reg Primate Res Ctr, Div Reprod Sci, Beaverton, OR USA
[2] Oregon Hlth Sci Univ, Oregon Reg Primate Res Ctr, Dept Obstet & Gynecol, Beaverton, OR USA
[3] Univ Pittsburgh, Pittsburgh Dev Ctr, Magee Womens Res Inst, Pittsburgh, PA USA
关键词
assisted reproduction; non-human primates; animal models; male infertility; spermatids; fertilization;
D O I
10.1016/S0015-0282(01)03281-2
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Objective: To establish pregnancies in rhesus monkeys using testicular sperm and elongated spermatids injected into oocytes. Design: Comparative animal study. Setting: Regional Primate Research Center. Animal(s): Prime, fertile rhesus monkeys. Intervention(s): Oocytes collected by laparoscopy from gonadotropin-stimulated female rhesus monkeys were injected with testicular sperm or elongated spermatids obtained from the testis of males. Cleavage stage embryos were transferred to surrogate females. Main Outcome Measure(s): Fertilization, ernbryo cleavage, and the establishment of pregnancies. Fertilization failures were fixed and processed for the detection or microtubules and chromatin configurations. Result(s): Fertilization, assessed by the presence of two pronuclei within 15 hours after injection, was 60% for intracytoplasmic sperm injection with testicular sperm and 47% for elongated spermatid injection. Fertilized zygotes co-cultured in Connaughts Medical Research Labs (CMRL) medium on a Buffalo Rat Liver cell monolayer resulted in hatched blastocysts after testicular sperm extraction-intracytoplasmic sperm injection and elongated spermatids. Embryos transferred at the 4- to 8-cell stage gave rise to three pregnancies: 2/3 from testicular sperm and 1/1 from an elongated spermatid. Three healthy infants were delivered by cesarean. Oocytes that failed to fertilize typically remained arrested in metaphase of meiosis. Conclusion(s): Testicular sperm and elongated spermatids can be used fur fertilization in the rhesus monkey resulting in live births. (C) 2002 by American Society for Reproductive Medicine.
引用
收藏
页码:794 / 801
页数:8
相关论文
共 61 条
[41]   MICROTUBULE CONFIGURATIONS DURING FERTILIZATION, MITOSIS, AND EARLY DEVELOPMENT IN THE MOUSE AND THE REQUIREMENT FOR EGG MICROTUBULE-MEDIATED MOTILITY DURING MAMMALIAN FERTILIZATION [J].
SCHATTEN, G ;
SIMERLY, C ;
SCHATTEN, H .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (12) :4152-4156
[42]   PREGNANCY AFTER FERTILIZATION WITH HUMAN TESTICULAR SPERMATOZOA [J].
SCHOYSMAN, R ;
VANDERZWALMEN, P ;
NIJS, M ;
SEGAL, L ;
SEGALBERTIN, G ;
GEERTS, L ;
VANROOSENDAAL, E ;
SCHOYSMAN, D .
LANCET, 1993, 342 (8881) :1237-1237
[43]   Distribution of spermatogenesis in the testicles of azoospermic men: the presence or absence of spermatids in the testes of men with germinal failure [J].
Silber, SJ ;
Nagy, Z ;
Devroey, P ;
Tournaye, H ;
Van Steirteghem, AC .
HUMAN REPRODUCTION, 1997, 12 (11) :2422-2428
[44]   Round spermatid injection [J].
Silber, SJ ;
Johnson, L ;
Verheyen, G ;
Van Steirteghem, A .
FERTILITY AND STERILITY, 2000, 73 (05) :897-900
[45]   THE PATERNAL INHERITANCE OF THE CENTROSOME, THE CELLS MICROTUBULE-ORGANIZING CENTER, IN HUMANS, AND THE IMPLICATIONS FOR INFERTILITY [J].
SIMERLY, C ;
WU, GJ ;
ZORAN, S ;
ORD, T ;
RAWLINS, R ;
JONES, J ;
NAVARA, C ;
GERRITY, M ;
RINEHART, J ;
BINOR, Z ;
ASCH, R ;
SCHATTEN, G .
NATURE MEDICINE, 1995, 1 (01) :47-52
[46]  
SIMERLY C, 1993, METHOD ENZYMOL, V225, P516
[47]   Ooplasmic injections of secondary spermatocytes for non-obstructive azoospermia [J].
Sofikitis, N ;
Mantzavinos, T ;
Loutradis, D ;
Yamamoto, Y ;
Tarlatzis, V ;
Miyagawa, I .
LANCET, 1998, 351 (9110) :1177-1178
[48]   Beneficial effects of electrical stimulation before round spermatid nuclei injections into rabbit oocytes on fertilization and subsequent embryonic development [J].
Sofikitis, NV ;
Toda, T ;
Miyagawa, I ;
Zavos, PM ;
Pasyianos, P ;
Mastelou, E .
FERTILITY AND STERILITY, 1996, 65 (01) :176-185
[49]   Clinical efficacy of spermatid conception: analysis using a new spermatid classification scheme [J].
Sousa, M ;
Barros, A ;
Takahashi, K ;
Oliveira, C ;
Silva, J ;
Tesarik, J .
HUMAN REPRODUCTION, 1999, 14 (05) :1279-1286
[50]   On-stage selection of single round spermatids using a vital, mitochondrion-specific fluorescent probe MitoTracker™ and high resolution differential interference contrast microscopy [J].
Sutovsky, P ;
Ramalho-Santos, J ;
Moreno, RD ;
Oko, R ;
Hewitson, L ;
Schatten, G .
HUMAN REPRODUCTION, 1999, 14 (09) :2301-2312