Inter-relationships between light and respiration in the control of ascorbic acid synthesis and accumulation in Arabidopsis thaliana leaves

被引:242
作者
Bartoli, Carlos G.
Yu, Jianping
Gomez, Facundo
Fernandez, Laura
McIntosh, Lee
Foyer, Christine H. [1 ]
机构
[1] Rothamsted Res, Crop Performance & Improvement Div, Harpenden AL5 2JQ, Herts, England
[2] Michigan State Univ, Dept Biochem & Mol Biol, E Lansing, MI 48824 USA
[3] Michigan State Univ, DOE, Plant Res Lab, E Lansing, MI 48824 USA
[4] Natl Univ La Plata, Inst Plant Physiol, INFIVE, Sch Agron, RA-1900 La Plata, Argentina
[5] Natl Univ La Plata, Inst Plant Physiol, INFIVE, Sch Nat Sci, RA-1900 La Plata, Argentina
基金
英国生物技术与生命科学研究理事会;
关键词
ascorbic acid; alternative oxidase; cytochrome c; light acclimation; mitochondria; respiration;
D O I
10.1093/jxb/erl005
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The effects of growth irradiance and respiration on ascorbic acid (AA) synthesis and accumulation were studied in the leaves of wild-type and transformed Arabidopsis thaliana with modified amounts of the mitochondrial alternative oxidase (AOX) protein. Plants were grown under low (LL; 50 mu mol photons m(-2) s(-1)), intermediate (IL; 100 mu mol photons m(-2) s(-1)), or high (HL; 250 mu mol photons m(-2) s-1) light. Increasing growth irradiance progressively elevated leaf AA content and hence the values of dark-induced disappearance of leaf AA, which were 11, 55, and 89 nmol AA lost g(-1) fresh weight h(-1), from LL-, IL-, and HL-grown leaves, respectively. When HL leaves were supplied with L-galactone-1,4-lactone (L-GalL; the precursor of AA), they accumulated twice as much AA and had double the maximal L-galactone-1,4-lactone dehydrogenase (L-GalLDH) activities of LL leaves. Growth under HL enhanced dehydroascorbate reductase and monodehydroascorbate reductase activities. Leaf respiration rates were highest in the HL leaves, which also had higher amounts of cytochrome c and cytochrome c oxidase (CCO) activities, as well as enhanced capacity of the AOX and CCO electron transport pathways. Leaves of the AOX-overexpressing lines accumulated more AA than wild-type or antisense leaves, particularly at HL. Intact mitochondria from AOX-overexpressing lines had higher AA synthesis capacities than those from the wild-type or antisense lines even though they had similar L-GaILDH activities. AOX antisense lines had more cytochrome c protein than wildtype or AOX-overexpressing lines. It is concluded that regardless of limitations on L-GalL synthesis by regulation of early steps in the AA synthesis pathway, the regulation of L-GaILDH activity via the interaction of light and respiratory controls is a crucial determinant of the overall ability of leaves to produce and accumulate AA.
引用
收藏
页码:1621 / 1631
页数:11
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