High-performance liquid chromatography determination of Zn-bacitracin in animal feed by post-column derivatization and fluorescence detection

被引：23

作者：

Capitan-Vallvey, LF

Titos, A

Checa, R

Navas, N

机构：

[1] Univ Granada, Fac Sci, Dept Analyt Chem, E-18071 Granada, Spain

[2] Ministerio Agr Pesca & Alimentac, Cent Vet Lab, E-18320 Granada, Spain

来源：

JOURNAL OF CHROMATOGRAPHY A | 2002年 / 943卷 / 02期

关键词：

food analysis; derivatization; LC; zinc; bacitracin;

D O I：

10.1016/S0021-9673(01)01442-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive and selective method is presented for the determination of Zn-Bacitracin in adulterated animal feed by reversed-phase ion-pair high-performance liquid chromatography and post-column derivatization with o-phthalaldehyde prior to fluorescence detection. The calibration function was estimated to be between 8.0 and 65.0 mg l(-1) of Zn-BC. The detection and quantification limits of the chromoatographic method were 2.5 and 7.5 mg l(-1), respectively. Using the extraction procedure of Zn-Bacitracin from the feedstuff that we recently proposed and applying this new chromatographic method, it was possible to detect this antibiotic at levels below 5 mg kg(-1) in different kinds of feedstuffs with a standard deviation less than 6.0%. (C) 2002 Published by Elsevier Science B.V.

引用

页码：227 / 234

页数：8