Clostridia: the importance of their exceptional substrate and metabolite diversity for biofuel and biorefinery applications

被引：308

作者：

Tracy, Bryan P. ^{[3
]}

Jones, Shawn W. ^{[1
,2
]}

Fast, Alan G. ^{[1
,2
]}

Indurthi, Dinesh C. ^{[1
,2
]}

Papoutsakis, Eleftherios T. ^{[1
,2
]}

机构：

[1] Univ Delaware, Dept Chem Engn, Mol Biotechnol Lab, Newark, DE 19711 USA

[2] Univ Delaware, Delaware Biotechnol Inst, Newark, DE 19711 USA

[3] Elcriton Inc, Newark, DE 19711 USA

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2012年 / 23卷 / 03期

关键词：

ACETOBUTYLICUM ATCC 824; COMPLETE GENOME SEQUENCE; PROPIONIC-ACID BACTERIA; EXPRESSION REPORTER SYSTEM; INTRON TARGETRON VECTOR; WOOD-LJUNGDAHL PATHWAY; BUTANOL PRODUCTION; SOLVENT PRODUCTION; GENE-EXPRESSION; CARBON-MONOXIDE;

D O I：

10.1016/j.copbio.2011.10.008

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Clostridia are anaerobic Firmicutes producing a large array of metabolites by utilizing simple and complex carbohydrates, such as cellulose, as well as CO2/H-2 or CO. Their exceptional substrate diversity is enhanced by their ability to produce a broad spectrum of chemicals that can be used as precursors to or directly as biofuels and industrial chemicals. Genetic and genomic tools are under intense development, and recent efforts to metabolically engineer clostridia demonstrate their potential for biofuel and biorefinery applications. Pathway engineering to combine established substrate-utilization programs, such as for cellulose, CO2/H2 or CO, with desirable metabolic programs could lead to modular design of strains suitable for many applications. Engineering complex phenotypes - aerotolerance, abolished sporulation, and tolerance to toxic chemicals - could lead to superior bioprocessing strains.

引用

页码：364 / 381

页数：18

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