Isolation of a new butanol-producing Clostridium strain: High level of hemicellulosic activity and structure of solventogenesis genes of a new Clostridium saccharobutylicum isolate

被引：31

作者：

Berezina, Oksana V. ^{[1
]}

Brandt, Agnieszka ^{[2
]}

Yarotsky, Sergey ^{[1
]}

Schwarz, Wolfgang H. ^{[2
]}

Zverlov, Vladimir V. ^{[2
,3
]}

机构：

[1] State Res Inst Genet & Select Ind Microorganisms, Moscow 117545, Russia

[2] Tech Univ Munich, Dept Microbiol, D-85350 Freising Weihenstephan, Germany

[3] Russian Acad Sci, Inst Mol Genet, Moscow 123182, Russia

来源：

SYSTEMATIC AND APPLIED MICROBIOLOGY | 2009年 / 32卷 / 07期

关键词：

ABE fermentation; Substrate utilization; Plant biomass hydrolysis; Solventogenesis; SOLVENT-PRODUCING CLOSTRIDIA; ACETOBUTYLICUM P262; BUTYRYL-COA; DEHYDROGENASE GENE; MOLECULAR ANALYSIS; SPACER-REGION; COENZYME-A; CLONING; FERMENTATION; EXPRESSION;

D O I：

10.1016/j.syapm.2009.07.005

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

New isolates of solventogenic bacteria exhibited high hemicellulolytic activity. They produced butanol and acetone with high selectivity for butanol (about 80% of butanol from the total solvent yield). Their 16S rDNA sequence was 99% identical to that of Clostridium saccharobutylicum. The genes responsible for the last steps of solventogenesis and encoding crotonase, butyryl-CoA dehydrogenase, electron-transport protein subunits A and B, 3-hydroxybutyryl-CoA dehydrogenase, alcohol dehydrogenase, CoA-transferase (subunits A and B), acetoacetate decarboxylase, and aldehyde dehydrogenase were identified in the new C saccharobutylicum strain Ox29 and cloned into Escherichia coli. The genes for crotonase, butyryl-CoA dehydrogenase, electron-transport protein subunits A and B, and 3-hydroxybutyryl-CoA dehydrogenase composed the bcs-operon. A monocistronic operon containing the alcohol dehydrogenase gene was located downstream of the bcs-operon. Genes for aldehyde dehydrogenase, CoA-transferase (subunits A and B), and acetoacetate decarboxylase composed the sol-operon. The gene sequences and the gene order within the sol- and bcs-operons of C saccharobutylicum Ox29 were most similar to those of Clostridium beijerinckii. The activity of some of the bcs-operon genes, expressed in heterologous E. coli, was determined. (C) 2009 Elsevier GmbH. All rights reserved.

引用

页码：449 / 459

页数：11

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