Proangiogenic and Prosurvival Functions of Glucose in Human Mesenchymal Stem Cells Upon Transplantation

被引：55

作者：

Deschepper, Mickael ^{[1
]}

Manassero, Mathieu ^{[1
]}

Oudina, Karim ^{[1
]}

Paquet, Joseph ^{[1
]}

Monfoulet, Laurent-Emmanuel ^{[1
]}

Bensidhoum, Morad ^{[1
]}

Logeart-Avramoglou, Delphine ^{[1
]}

Petite, Herve ^{[1
]}

机构：

[1] Univ Paris 07, Fac Med Lariboisiere St Louis, Lab Bioingn & Biomecan Osteoarticulaire, B2OA,CNRS,UMR 7052, Paris, France

来源：

STEM CELLS | 2013年 / 31卷 / 03期

关键词：

Human mesenchymal stem cells; Marrow stromal cells; Anoxia; Glucose; Ischemia; Angiogenesis; IN-VITRO; HYPOXIA; INHIBITION; DIFFERENTIATION; ISCHEMIA; DEATH;

D O I：

10.1002/stem.1299

中图分类号：

Q813 [细胞工程];

学科分类号：

100113 [医学细胞生物学];

摘要：

A major limitation in the development of cellular therapies using human mesenchymal stem cells (hMSCs) is cell survival post-transplantation. In this study, we challenged the current paradigm of hMSC survival, which assigned a pivotal role to oxygen, by testing the hypothesis that exogenous glucose may be key to hMSC survival. We demonstrated that hMSCs could endure sustained near-anoxia conditions only in the presence of glucose. In this in vitro cell model, the protein expressions of Hif-1 alpha and angiogenic factors were upregulated by the presence of glucose. Ectopically implanted tissue constructs supplemented with glucose exhibited four- to fivefold higher viability and were more vascularized compared to those without glucose at day 14. These findings provided the first direct in vitro and in vivo demonstration of the proangiogenic and prosurvival functions of glucose in hMSC upon transplantation and identified glucose as an essential component of the ideal scaffold for transplanting stem cells. STEM CELLS 2013;31:526-535

引用

页码：526 / 535

页数：10

共 24 条

[1]

Hypoxia induces vascular endothelial growth factor gene transcription in human osteoblast-like cells through the hypoxia-inducible factor-2α [J].