Detection of Cryptosporidium in miniaturised fluidic devices

被引：44

作者：

Bridle, Helen ^{[1
]}

Kersaudy-Kerhoas, Maiwenn ^{[2
]}

Miller, Brian ^{[1
]}

Gavriilidou, Despoina ^{[1
]}

Katzer, Frank ^{[3
]}

Innes, Elisabeth A. ^{[3
]}

Desmulliez, Marc P. Y. ^{[2
]}

机构：

[1] Univ Edinburgh, Edinburgh, Midlothian, Scotland

[2] Heriot Watt Univ, MicroSyst Engn Ctr MISEC, Edinburgh, Midlothian, Scotland

[3] Moredun Res Inst, Edinburgh EH17 7JH, Midlothian, Scotland

来源：

WATER RESEARCH | 2012年 / 46卷 / 06期

基金：

英国生物技术与生命科学研究理事会; 英国工程与自然科学研究理事会;

关键词：

Parasite; Cryptosporidium; Detection; Miniaturisation; Microfluidics; SEQUENCE-BASED AMPLIFICATION; REAL-TIME DETECTION; LABEL-FREE DETECTION; MICROFABRICATED DEVICES; MICROFLUIDIC DEVICE; GIARDIA-LAMBLIA; MICROBIAL-CELLS; RAPID DETECTION; PARVUM OOCYST; PEMC SENSORS;

D O I：

10.1016/j.watres.2012.01.010

中图分类号：

X [环境科学、安全科学];

学科分类号：

083001 [环境科学];

摘要：

Contamination of drinking water with the protozoan pathogen, Cryptosporidium, represents a serious risk to human health due to the low infectious dose and the resistance of this parasite to chlorine disinfection. Therefore, several countries have legislated for the frequent monitoring of drinking water for Cryptosporidium presence. Existing approved monitoring protocols are however time-consuming and do not provide essential information on the species, virulence or viability of detected oocysts. Rapid, more information-rich and automatable systems for Cryptosporidium detection are highly sought-after, and numerous miniaturised devices have been developed to address this need. This review article aims to summarise the state-of-the-art and compare the performance of these systems in terms of detection limit, ability to determine species, viability and performance in the presence of interferents. Finally, conclusions are drawn with regard to the most promising methods and directions of future research. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：1641 / 1661

页数：21

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[2]

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