The choice of reference cell in the analysis of kinetic data using BIAcore

被引：17

作者：

Ober, RJ

Ward, ES

机构：

[1] Univ Texas, SW Med Ctr, Ctr Canc Immunobiol, Dallas, TX 75235 USA

[2] Univ Texas, SW Med Ctr, Ctr Immunol, Dallas, TX 75235 USA

[3] Univ Texas, Ctr Engn Math EC35, Richardson, TX 75083 USA

来源：

ANALYTICAL BIOCHEMISTRY | 1999年 / 271卷 / 01期

关键词：

kinetic constants; surface plasmon resonance; BIAcore; reference cell;

D O I：

10.1006/abio.1999.4129

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An important step in the analysis of sensorgram data for BIAcore experiments is the subtraction of reference cell data to remove the effects of the hulk shift on the sensorgram of interest. It is shown that this step can introduce errors in the measured kinetic constants. This phenomenon is investigated both theoretically and with experimental data. (C) 1999 Academic Press.

引用

收藏

页码：70 / 80

页数：11

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