Reliable determination of binding affinity and kinetics using surface plasmon resonance biosensors

被引：138

作者：

Schuck, P

机构：

[1] Molec. Intrac. Rsrc., Biomed. E., Natl. Center for Research Resources, National Institutes of Health, Bethesda, MD 20892-5766

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 1997年 / 8卷 / 04期

关键词：

D O I：

10.1016/S0958-1669(97)80074-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Progress has been made in the identification of experimental and analytical procedures that allow for a more reliable determination of equilibrium and kinetic constants, Possible origins of the frequently observed deviations of the measured binding progress from that expected for chemical binding of pseudo-first order, and appropriate experimental controls have been proposed. Improved analytical approaches include the application of global analysis and analytical corrections for the influence of mass transport.

引用

页码：498 / 502

页数：5

共 43 条

[1] DIFFUSION-CONTROLLED MACROMOLECULAR INTERACTIONS [J].

BERG, OG ;

VONHIPPEL, PH .

ANNUAL REVIEW OF BIOPHYSICS AND BIOPHYSICAL CHEMISTRY, 1985, 14 :131-160

[2] Studies of protein interactions by biosensor technology: An alternative approach to the analysis of sensorgrams deviating from pseudo-first-order kinetic behavior [J].

Bowles, MR ;

Hall, DR ;

Pond, SM ;

Winzor, DJ .

ANALYTICAL BIOCHEMISTRY, 1997, 244 (01) :133-143

[3]

Crank J., 1975, The Mathematics of Diffusion, V2nd ed.

[4] A mutational analysis of the binding of two different proteins to the same antibody [J].

DallAcqua, W ;

Goldman, ER ;

Eisenstein, E ;

Mariuzza, RA .

BIOCHEMISTRY, 1996, 35 (30) :9667-9676

[5] THE BIOPHYSICS OF LIGAND-RECEPTOR INTERACTIONS [J].

DELISI, C .

QUARTERLY REVIEWS OF BIOPHYSICS, 1980, 13 (02) :201-230

[6] KINETICS OF PROTEIN-PROTEIN INTERACTIONS AT THE SURFACE OF AN OPTICAL BIOSENSOR [J].

EDWARDS, PR ;

GILL, A ;

POLLARDKNIGHT, DV ;

HOARE, M ;

BUCKLE, PE ;

LOWE, PA ;

LEATHERBARROW, RJ .

ANALYTICAL BIOCHEMISTRY, 1995, 231 (01) :210-217

[7]

Fisher Robert J., 1994, Methods (Orlando), V6, P121, DOI 10.1006/meth.1994.1015

[8]

Fisher Robert J., 1994, Current Opinion in Biotechnology, V5, P389, DOI 10.1016/0958-1669(94)90047-7

[9] Optical evanescent wave methods for the study of biomolecular interactions [J].

Garland, PB .

QUARTERLY REVIEWS OF BIOPHYSICS, 1996, 29 (01) :91-117

[10] STABLE CHELATING LINKAGE FOR REVERSIBLE IMMOBILIZATION OF OLIGOHISTIDINE TAGGED PROTEINS IN THE BIACORE SURFACE-PLASMON RESONANCE DETECTOR [J].

GERSHON, PD ;

KHILKO, S .

JOURNAL OF IMMUNOLOGICAL METHODS, 1995, 183 (01) :65-76

← 1 2 3 4 5 →