Reliable determination of binding affinity and kinetics using surface plasmon resonance biosensors

被引：138

作者：

Schuck, P

机构：

[1] Molec. Intrac. Rsrc., Biomed. E., Natl. Center for Research Resources, National Institutes of Health, Bethesda, MD 20892-5766

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 1997年 / 8卷 / 04期

关键词：

D O I：

10.1016/S0958-1669(97)80074-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Progress has been made in the identification of experimental and analytical procedures that allow for a more reliable determination of equilibrium and kinetic constants, Possible origins of the frequently observed deviations of the measured binding progress from that expected for chemical binding of pseudo-first order, and appropriate experimental controls have been proposed. Improved analytical approaches include the application of global analysis and analytical corrections for the influence of mass transport.

引用

页码：498 / 502

页数：5

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