Phosphonate monolayers functionalized by silver thiolate species as antibacterial nanocoatings on titanium and stainless steel

被引：66

作者：

Amalric, Julien ^{[1
]}

Mutin, P. Hubert ^{[1
]}

Guerrero, Gilles ^{[1
]}

Ponche, Arnaud ^{[2
]}

Sotto, Albert ^{[3
]}

Lavigne, Jean-Philippe ^{[3
]}

机构：

[1] Univ Montpellier 2, CNRS UM2, UMR 5253, Inst Charles Gerhardt Montpellier, F-34095 Montpellier 5, France

[2] CNRS, UPR 9069, Inst Chim Surfaces & Interfaces Mulhouse, Mulhouse, France

[3] Univ Montpellier 1, Espri 26, Inst Natl Sante & Rech Med, UFR Med, Nimes, France

来源：

JOURNAL OF MATERIALS CHEMISTRY | 2009年 / 19卷 / 01期

关键词：

SELF-ASSEMBLED MONOLAYERS; STAPHYLOCOCCUS-AUREUS; BIOFILM FORMATION; ALKYLPHOSPHONIC ACID; SURFACE MODIFICATION; COUPLING MOLECULES; ESCHERICHIA-COLI; HYBRID MATERIALS; THIN-FILMS; IN-VITRO;

D O I：

10.1039/b813344a

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Titanium and stainless steel substrates were modified by grafting with mercaptododecylphosphonic acid (MDPA) followed by reaction with silver nitrate (AgNO3), in order to investigate the potential of phosphonate self-assembled monolayers functionalized by silver thiolate species as antibacterial nanocoatings for inorganic biomaterials. The samples were characterized by Fourier transform infrared (FTIR) spectroscopy in grazing-incidence mode, water contact angle measurements, and X-ray photoelectron spectroscopy (XPS). The influence of the surface modification on bacterial adhesion and biofilm growth was investigated in vitro using Escherichia coli, Pseudomonas aeruginosa, Staphylococcus epidermidis, and Staphylococcus aureus strains. The stability of the monolayer in blood-mimicking medium was examined. Despite their very low silver content, MDPA + AgNO3 monolayers strongly decreased bacterial adhesion (> 99.9% reduction in the number of viable adherent bacteria) and biofilm formation in comparison to the bare substrates.

引用

页码：141 / 149

页数：9

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