Simultaneous detection of Pseudomonas fragi, P. lundensis, and P. putida from meat by use of a multiplex PCR assay targeting the carA gene

被引：96

作者：

Ercolini, Danilo ^{[1
]}

Russo, Federica ^{[1
]}

Blaiotta, Giuseppe ^{[1
]}

Pepe, Olimpia ^{[1
]}

Mauriello, Gianluigi ^{[1
]}

Villani, Francesco ^{[1
]}

机构：

[1] Univ Naples Federico II, Dipartimento Sci Alimenti, I-80055 Portici, NA, Italy

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2007年 / 73卷 / 07期

关键词：

16S-23S RDNA SPACER; 16S RDNA; SPOILAGE; CHEESE; MILK; MICROFLORA; DIVERSITY; SEQUENCES; FRESH; WATER;

D O I：

10.1128/AEM.02603-06

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Species-specific primers and a multiplex PCR assay were developed for the simultaneous identification and differentiation of Pseudomonas fragi, P. lundensis, and P. putida based on the coamplification of different portions of the small subunit of the carbamoyl phosphate synthase gene (carA). The carA multiplex PCR was used to detect the presence of the three Pseudomonas species from beef, chicken, and pork samples and proved to be effective in showing their evolution during the storage of meat.

引用

页码：2354 / 2359

页数：6