Uncoupling protein and alternative oxidase of Dictyostelium discoideum:: occurrence, properties and protein expression during vegetative life and starvation-induced early development

被引：41

作者：

Jarmuszkiewicz, W

Behrendt, M

Navet, R

Sluse, FE ^{[1
]}

机构：

[1] Univ Liege, Inst Chem B6, Dept Life Sci, Lab Bioenerget, B-4000 Liege, Belgium

[2] Adam Mickiewicz Univ Poznan, Dept Bioenerget, Inst Mol Biol & Biotechnol, PL-61704 Poznan, Poland

来源：

FEBS LETTERS | 2002年 / 532卷 / 03期

关键词：

mitochondrion; alternative oxidase; uncoupling protein; development; Dictyostelium discoideum;

D O I：

10.1016/S0014-5793(02)03734-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study we show that mitochondria of Dictyostelium discoideum contain both alternative oxidase (AOX) and uncoupling protein (UCP). AOX was stimulated by purine mononucleoside and was monomeric. UCP was stimulated by free fatty acids and was poorly sensitive to GTP. Both proteins collaborated in energy dissipation when activated together. AOX expression in free-living ameboid cells decreased strongly from exponential to stationary phase of growth but much less during starvation-induced aggregation. In contrast, UCP expression was constant in all conditions indicating permanent need. Our results suggest that AOX could play a role in cell differentiation, mainly by protecting prespore cells from programmed cell death. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：459 / 464

页数：6

共 36 条

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