Fluorescent polyelectrolytes as protein sensors

被引:57
作者
Ambade, Ashootosh Vasant [1 ]
Sandanaraj, Britto Selvaraj [1 ]
Klaikherd, Akamol [1 ]
Thayumanavan, S. [1 ]
机构
[1] Univ Massachusetts, Dept Chem, Amherst, MA 01003 USA
关键词
conjugated polymers; polyelectrolytes; protein sensors; fluorescence quenching; Stern-Volmer quenching constant; FRET; enzyme kinetics; metalloproteins; amyloid fibril formation;
D O I
10.1002/pi.2185
中图分类号
O63 [高分子化学(高聚物)];
学科分类号
070305 ; 080501 ; 081704 ;
摘要
This review discusses recent advances in protein sensing using fluorescent polyelectrolytes that are mainly water-soluble conjugated polymers. A quencher-labeled substrate or fluorophore-labeled substrate is generally used as a probe. In the presence of an enzyme, the linker between substrate and quencher/fluorophore is cleaved and fluorescence of the polymer is either 'turned on' or 'turned off. Fluorescence behavior of these conjugated polymers is highly sensitive to conformation of the polymeric chains. Since upon binding with proteins the conformation is perturbed and fluorescence is affected, these polyelectrolytes have been used to study conformational changes in proteins. The conformation-dependent fluorescence is also a limitation for these sensors in some cases and non-conjugated polyelectrolytes have been shown to provide an alternative. (C) 2006 Society of Chemical Industry.
引用
收藏
页码:474 / 481
页数:8
相关论文
共 57 条
[1]   Fluorescence superquenching of conjugated polyelectrolytes: applications for biosensing and drug discovery [J].
Achyuthan, KE ;
Bergstedt, TS ;
Chen, L ;
Jones, RM ;
Kumaraswamy, S ;
Kushon, SA ;
Ley, KD ;
Lu, L ;
McBranch, D ;
Mukundan, H ;
Rininsland, F ;
Shi, X ;
Xia, W ;
Whitten, DG .
JOURNAL OF MATERIALS CHEMISTRY, 2005, 15 (27-28) :2648-2656
[2]   Multicolored electrochromism polymers: Structures and devices [J].
Argun, AA ;
Aubert, PH ;
Thompson, BC ;
Schwendeman, I ;
Gaupp, CL ;
Hwang, J ;
Pinto, NJ ;
Tanner, DB ;
MacDiarmid, AG ;
Reynolds, JR .
CHEMISTRY OF MATERIALS, 2004, 16 (23) :4401-4412
[3]   LIGHT-EMITTING-DIODES FABRICATED WITH CONJUGATED POLYMERS - RECENT PROGRESS [J].
BAIGENT, DR ;
GREENHAM, NC ;
GRUNER, J ;
MARKS, RN ;
FRIEND, RH ;
MORATTI, SC ;
HOLMES, AB .
SYNTHETIC METALS, 1994, 67 (1-3) :3-10
[4]  
Banner C.D., 1992, PROTEASES PROTEASE I
[5]   Invertible amphiphilic homopolymers [J].
Basu, S ;
Vutukuri, DR ;
Shyamroy, S ;
Sandanaraj, BS ;
Thayumanavan, S .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (32) :9890-9891
[6]   Transmissible spongiform encephalopathies, amyloidoses and yeast prions: Common threads? [J].
Caughey, B .
NATURE MEDICINE, 2000, 6 (07) :751-754
[7]   Highly sensitive biological and chemical sensors based on reversible fluorescence quenching in a conjugated polymer [J].
Chen, LH ;
McBranch, DW ;
Wang, HL ;
Helgeson, R ;
Wudl, F ;
Whitten, DG .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (22) :12287-12292
[8]   Tuning the properties of conjugated polyelectrolytes through surfactant complexation [J].
Chen, LH ;
Xu, S ;
McBranch, D ;
Whitten, D .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2000, 122 (38) :9302-9303
[9]   Saccharide detection based on the amplified fluorescence quenching of a water-soluble poly(phenylene ethynylene) by a boronic acid functionalized benzyl viologen derivative [J].
DiCesare, N ;
Pinto, MR ;
Schanze, KS ;
Lakowicz, JR .
LANGMUIR, 2002, 18 (21) :7785-7787
[10]   Electrochemical DNA sensors [J].
Drummond, TG ;
Hill, MG ;
Barton, JK .
NATURE BIOTECHNOLOGY, 2003, 21 (10) :1192-1199