Chemiluminescence determination of ultramicro DNA with a flow-injection method

被引：34

作者：

Chen, H ^{[1
]}

Zhou, M ^{[1
]}

Jin, XY ^{[1
]}

Song, YM ^{[1
]}

Zhang, ZY ^{[1
]}

Ma, YJ ^{[1
]}

机构：

[1] NW Normal Univ, Inst Chem, Lanzhou 730070, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 2003年 / 478卷 / 01期

关键词：

chemiluminescence; flow-injection analysis; calf thymus DNA; herring sperm DNA; cerium(IV); rhodamine B; DNA;

D O I：

10.1016/S0003-2670(02)01477-0

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A high sensitive flow-injection chemiluminescence method for determination of calf thymus DNA and herring sperm DNA has been developed. The method is based on the chemiluminescence reaction of Rhodamine B-cerium(IV)-thermally denatured DNAs in sulfuric acid media. The proposed procedure allows quantitation of DNAs in the range 2.6 x 10(-5) to 0.26 mug ml(-1) for calf thymus DNA and 5.0 x 10(-8) to 5.0 x 10(-5) mug ml(-1) for herring sperm DNA with correlation coefficients 0.9998 and 0.9996 (both n = 11), respectively. The detection limits (3sigma) are 6.5 x 10(-6) mug ml(-1) for calf thymus DNA and 4.3 x 10(-8) mug ml(-1) for herring sperm DNA. The possible mechanism of chemiluminescence in the system is discussed. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：31 / 36

页数：6

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