Simple and sensitive assay for nucleic acids by use of Rayleigh light scattering technique with rhodamine B

被引：51

作者：

Liu, Y ^{[1
]}

Ma, CQ ^{[1
]}

Lie, KA ^{[1
]}

Tong, SY ^{[1
]}

机构：

[1] Peking Univ, Coll Chem & Mol Engn, Inst Analyt Chem, Beijing 100871, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 1999年 / 379卷 / 1-2期

基金：

中国国家自然科学基金;

关键词：

Rayleigh light scattering; nucleic acids; DNA; rhodamine B;

D O I：

10.1016/S0003-2670(98)00679-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Based on the enhancement of Rayleigh light scattering of rhodamine B by nucleic acids under acidic condition at 378 nm, a new method for determination of nucleic acids in aqueous solutions has been developed. This assay has a wide linear range for calf thymus DNA (0.10-16.0 mu g ml(-1)). Besides its high sensitivity, it has some other advantages: rapidity of reaction (<2 min), simplicity of operation (one-step assay), commonality of spectrofluorimeter and reagents, stability of complexes formed and reproducibility. Under the experimental conditions, there is little or no interference from proteins, nucleosides and most metal ions. Interference by some metal ions, surfactants and salts can be minimized by dilution. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：39 / 44

页数：6

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