Improved latex agglutination test for detection of antibodies in serum and cerebrospinal fluid of Trypanosoma brucei gambiense infected patients

被引：35

作者：

Büscher, P

Lejon, V

Magnus, E

Van Meirvenne, N

机构：

[1] Univ Antwerp, Inst Trop Med, Dept Parasitol, B-2000 Antwerp, Belgium

[2] Univ Instelling Antwerp, Dept Biochem, B-2610 Wilrijk, Belgium

来源：

ACTA TROPICA | 1999年 / 73卷 / 01期

关键词：

sleeping sickness; Trypanosoma brucei gambiense; serodiagnosis; latex agglutination; serum; cerebrospinal fluid;

D O I：

10.1016/S0001-706X(99)00009-1

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

A rapid latex agglutination test (LATEX/T. b. gambiense) for detection of antibodies in patients infected with Trypanosoma brucei gambiense is presented. The reagent is coated with a mixture of three variable surface antigens of bloodstream form trypanosomes. Two hundred and forty sera and 79 CSF samples from patients with parasitologically confirmed trypanosome infection along with 173 sera and 38 CSF samples from non-trypanosomiasis patients have been tested. At 1:16 serum dilution, test specificity was 99%, while sensitivity ranged from 83.8 to 100% depending on the geographical origin of the samples. Undiluted CSF samples from non-trypanosomiasis and from first stage patients scored negative while 42 out of 66 CSF samples from second stage patients were positive. Stability and reproducibility of the lyophilized reagent were excellent. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：11 / 20

页数：10

共 14 条

[11] TrypTect CIATT(R) - a card indirect agglutination trypanosomiasis test for diagnosis of Trypanosoma brucei gambiense and T-b-rhodesiense infections [J].