Capsaicin-sensitive vagal fibres and 5-HT3-, gastrin releasing peptide- and cholecystokinin A-receptors are involved in distension-induced inhibition of gastric emptying in the rat

被引:24
作者
Bozkurt, A [1 ]
Oktar, BK [1 ]
Kurtel, H [1 ]
Alican, I [1 ]
Coskun, T [1 ]
Yegen, BÇ [1 ]
机构
[1] Marmara Univ, Sch Med, Dept Physiol, TR-81326 Istanbul, Turkey
关键词
distension; GRP; 5-HT3; CCK; capsaicin; mechanoreceptor;
D O I
10.1016/S0167-0115(99)00050-6
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The present study was undertaken to investigate how the activation of gastric mechanoreceptors by distension of the stomach in conscious gastric fistula rats influences gastric emptying; and the roles of capsaicin sensitive vagal afferent fibres and the 5-HT3, GRP and CCK-A receptors involved in mediating these responses. To activate mechanoreceptors by non-nutrient dependent pathways, methylcellulose in saline was used to distend the stomach (5 cm H2O) and the subsequent emptying of saline was examined immediately, and at 3, 5 and 10 min following distension. Prior distension delayed the subsequent emptying of saline instilled into the stomach compared with non-distended controls (2.28+/-0.09 m1/5 min; P<0.001). Topical application of capsaicin, completely abolished the distension-induced inhibition of gastric emptying when compared with vehicle treated rats (2.82+/-0.09 vs. 2.38+/-0.04 m1/5 min; P < 0.001). Peripheral administration of a CRP antagonist (2258 U89UJ, 1 mg/kg), and a 5-HT3 antagonist (BRL4369UA, 50 mu g/kg) significantly reversed (2.56+/-0.14 m1/5 min; P < 0.05 and 2.61+/-0.07 m1/5 min; P < 0.01; respectively) the delay in gastric emptying induced by distension. When the rats were treated with the CCK-A antagonist, gastric emptying of saline following distension was also significantly facilitated (2.56+/-0.07 m1/5 min; P < 0.001). In contrast, the CCK-B/gastrin receptor antagonist had no significant effect pn the distension induced delay in gastric emptying (1.95+/-0.12 m1/5 min). The present results suggest that gastric distension in conscious gastric fistula rats delays gastric emptying by activating capsaicin-sensitive extrinsic afferent nerve fibres. Moreover, the results also indicate that distension-induced mechanisms involve GRP, 5-HT3 and CCK-A receptors, but not CCK-B receptors. (C) 1999 Elsevier Science BN. All rights reserved.
引用
收藏
页码:81 / 86
页数:6
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