Bilirubin-Cu(II) complex degrades DNA

被引：25

作者：

Asad, SF ^{[1
]}

Singh, S ^{[1
]}

Ahmad, A ^{[1
]}

Hadi, SM ^{[1
]}

机构：

[1] Aligarh Muslim Univ, Dept Biochem, Fac Life Sci, Aligarh 202002, Uttar Pradesh, India

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 1999年 / 1428卷 / 2-3期

关键词：

bilirubin; copper; endogenous DNA damage; prooxidant; oxygen radical;

D O I：

10.1016/S0304-4165(99)00075-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

It has recently been reported that bilirubin forms a complex with Cu(II). In this paper we show that the formation of the complex results in the reduction of Cu(II) to Cu(I) and the redox cycling of the metal gives rise to the formation of reactive oxygen species, particularly hydroxyl radical. The bilirubin-Cu(II) complex causes strand breakage in calf thymus DNA and supercoiled plasmid DNA. Cu(I) was shown to be an essential intermediate in the DNA cleavage reaction by using the Cu(I) specific sequestering reagent neocuproine. Bilirubin-Cu(II) produced hydroxyl radical and the involvement of active oxygen species was established by the inhibition of DNA breakage by various oxygen radical quenchers. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：201 / 208

页数：8

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