Development and validation of an oligonucleotide microarray for detection of multiple virulence and antimicrobial resistance genes in Escherichia coli

被引：79

作者：

Bruant, Guillaume

Maynard, Christine

Bekal, Sadjia

Gaucher, Isabelle

Masson, Luke

Brousseau, Roland

Harell, Jose

机构：

[1] Univ Montreal, Fac Med Vet, Grp Rech Malad Infect Porc, St Hyacinthe, PQ J2S 7C6, Canada

[2] Natl Res Council Canada, Biotechnol Res Inst, Montreal, PQ H4P 2R2, Canada

[3] Inst Natl Sante Publ Quebec, Ste Anne De Bellevue, PQ H9X 3R5, Canada

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2006年 / 72卷 / 05期

关键词：

D O I：

10.1128/AEM.72.5.3780-3784.2006

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An oligonucleotide microarray detecting 189 Escherichia coli virulence genes or markers and 30 antimicrobial resistance genes was designed and validated using DNA from known reference strains. This microarray was confirmed to be a powerful diagnostic tool for monitoring emerging E. coli pathotypes and antimicrobial resistance, as well as for environmental, epidemiological, and phylogenetic studies including the evaluation of genome plasticity.

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页码：3780 / 3784

页数：5

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