Transgenic livestock made easy

被引:24
作者
Whitelaw, CBA [1 ]
机构
[1] Roslin Inst, Dept Gene Express & Dev, Roslin EH25 9PS, Midlothian, Scotland
关键词
D O I
10.1016/j.tibtech.2004.02.005
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The ability to transfer (trans)genes into the germline of domestic animals is both exciting and challenging. Many believe that this biotechnology has huge potential to benefit both farm animals and humans. However, despite several encouraging projects, overall transgenesis in livestock has been severely restricted by the gross inefficiencies associated with the methodology. Simply put, the generation of transgenic livestock is very costly. In turn, this has limited the range of studies using transgenic farm animals. A recent paper, by describing what can only be termed as the dramatically efficient generation of transgenic pigs, changes everything. We can now look forward to an expansion of the use of transgenic livestock, quicker results and more progress towards realizing the exciting potential that transgenic livestock offer.
引用
收藏
页码:157 / 159
页数:3
相关论文
共 16 条
[1]   Transgenic cattle produced by reverse-transcribed gene transfer in oocytes [J].
Chan, AWS ;
Homan, EJ ;
Ballou, LU ;
Burns, JC ;
Bremel, RD .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (24) :14028-14033
[2]   A future for transgenic livestock [J].
Clark, J ;
Whitelaw, B .
NATURE REVIEWS GENETICS, 2003, 4 (10) :825-833
[3]   Deletion of the α(1,3)galactosyl transferase (GGTA1) gene and the prion protein (PrP) gene in sheep [J].
Denning, C ;
Burl, S ;
Ainslie, A ;
Bracken, J ;
Dinnyes, A ;
Fletcher, J ;
King, T ;
Ritchie, M ;
Ritchie, WA ;
Rollo, M ;
de Sousa, P ;
Travers, A ;
Wilmut, I ;
Clark, AJ .
NATURE BIOTECHNOLOGY, 2001, 19 (06) :559-562
[4]   PRODUCTION OF TRANSGENIC RABBITS, SHEEP AND PIGS BY MICROINJECTION [J].
HAMMER, RE ;
PURSEL, VG ;
REXROAD, CE ;
WALL, RJ ;
BOLT, DJ ;
EBERT, KM ;
PALMITER, RD ;
BRINSTER, RL .
NATURE, 1985, 315 (6021) :680-683
[5]   Towards an ovine model of cystic fibrosis [J].
Harris, A .
HUMAN MOLECULAR GENETICS, 1997, 6 (13) :2191-2193
[6]   Efficient transgenesis in farm animals by lentiviral vectors [J].
Hofmann, A ;
Kessler, B ;
Ewerling, S ;
Weppert, M ;
Vogg, B ;
Ludwig, H ;
Stojkovic, M ;
Boelhauve, M ;
Brem, G ;
Wolf, E ;
Pfeifer, A .
EMBO REPORTS, 2003, 4 (11) :1054-1060
[7]   Efficient production by sperm-mediated gene transfer of human decay accelerating factor (hDAF) transgenic pigs for xenotransplantation [J].
Lavitrano, M ;
Bacci, ML ;
Forni, M ;
Lazzereschi, D ;
Di Stefano, C ;
Fioretti, D ;
Giancotti, P ;
Marfé, G ;
Pucci, L ;
Renzi, L ;
Wang, HJ ;
Stoppacciaro, A ;
Stassi, G ;
Sargiacomo, M ;
Sinibaldi, P ;
Turchi, V ;
Giovannoni, R ;
Casa, GD ;
Seren, E ;
Rossi, G .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (22) :14230-14235
[8]   Germline transmission and tissue-specific expression of transgenes delivered by lentiviral vectors [J].
Lois, C ;
Hong, EJ ;
Pease, S ;
Brown, EJ ;
Baltimore, D .
SCIENCE, 2002, 295 (5556) :868-872
[9]   Effect of DNA concentration on transgenesis rates in mice and pigs [J].
Nottle, MB ;
Haskard, KA ;
Verma, PJ ;
Du, ZT ;
Grupen, CG ;
McIlfatrick, SM ;
Ashman, RJ ;
Harrison, SJ ;
Barlow, H ;
Wigley, PL ;
Lyons, IG ;
Cowan, PJ ;
Crawford, RJ ;
Tolstoshev, PL ;
Pearse, MJ ;
Robins, AJ ;
d'Apice, AJF .
TRANSGENIC RESEARCH, 2001, 10 (06) :523-531
[10]   Transgenesis by lentiviral vectors: Lack of gene silencing in mammalian embryonic stem cells and preimplantation embryos [J].
Pfeifer, A ;
Ikawa, M ;
Dayn, Y ;
Verma, IM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (04) :2140-2145