Expression of the INU2 gene for an endoinulinase of Aspergillus ficuum in Saccharomyces cerevisiae

被引：30

作者：

Kim, HS

Lee, DW

Ryu, EJ

Uhm, TB

Yang, MS

Kim, JB

Chae, KS ^{[1
]}

机构：

[1] Chonbuk Natl Univ, Fac Biol Sci, Chonbuk 561756, South Korea

[2] Chonbuk Natl Univ, Inst Mol Biol & Genet, Chonbuk 561756, South Korea

[3] Sam Bae Co, R&D Ctr, Chonbuk 570810, South Korea

来源：

BIOTECHNOLOGY LETTERS | 1999年 / 21卷 / 07期

关键词：

Aspergillus ficuum; endoinulinase; gene : INU2; Kluyveromyces marxianus; overexpression; Saccharomyces cerevisiae;

D O I：

10.1023/A:1005567403830

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The INU2 gene encoding an endoinulinase of Aspergillus ficuum was expressed by the Kluyveromyces marxianus INU1 promoter in a SUC2-deleted Saccharomyces cerevisiae to produce the endoinulinase preparation free of an exoinulinase and an extracellular invertase in the culture medium. A recombinant yeast strain produced the sufficient amount of the enzyme to make a halo around its colony, when inulin was included in the medium.

引用

页码：621 / 623

页数：3

共 9 条

[1]

[Anonymous], 1982, ENZYME MICROB TECH

[2]

BERGKAMP RJM, 1993, APPL MICROBIOL BIOT, V40, P309

[3] TRANSFORMATION OF INTACT YEAST-CELLS TREATED WITH ALKALI CATIONS [J].