Inulin synthesis by a combination of purified fructosyltransferases from tubers of Helianthus tuberosus

被引：74

作者：

Luscher, M ^{[1
]}

Erdin, C ^{[1
]}

Sprenger, N ^{[1
]}

Hochstrasser, U ^{[1
]}

Boller, T ^{[1
]}

Wiemken, A ^{[1
]}

机构：

[1] UNIV BASEL, DEPT BOT, CH-4056 BASEL, SWITZERLAND

来源：

FEBS LETTERS | 1996年 / 385卷 / 1-2期

关键词：

fructan; fructan-fructan; 1-fructosyltransferase; Helianthus tuberosus; inulin; sucrose-sucrose;

D O I：

10.1016/0014-5793(96)00343-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sucrose-sucrose 1-fructosyltransferase (1-SST) was purified 100-fold from tubers of Helianthus tuberosus L. The purified enzyme was essentially devoid of invertase activity and could be separated by isoelectric focusing into five isoforms which all were composed of two subunits (59 and 26 kDa). Fructan-fructan 1-fructosyltransferase (1-FFT) was purified from the same source [M. Luscher et al. (1993) New Phytologist 123, 437-442). When incubated individually with sucrose, 1-FFT was inactive while 1-SST formed isokestose (trimer) and, upon prolonged incubation, some nystose (tetramer). When a combination of the two enzymes was incubated with sucrose, a series of oligofructosides with a degree of polymerization of up to 20 was formed. Amino acid sequences of tryptic peptide fragments from both 1-SST and 1-FFT indicate that these enzymes are highly homologous with plant invertases.

引用

页码：39 / 42

页数：4

共 21 条

[1] CLONING AND SEQUENCE OF CDNAS FOR AN INTRACELLULAR ACID INVERTASE FROM ETIOLATED HYPOCOTYLS OF MUNG BEAN AND EXPRESSION OF THE GENE DURING GROWTH OF SEEDLINGS [J].