Rapid selection of transgenic C. elegans using antibiotic resistance

被引：58

作者：

Semple, Jennifer I. ^{[1
]}

Garcia-Verdugo, Rosa ^{[1
]}

Lehner, Ben ^{[1
,2
]}

机构：

[1] UPF, Genom Regulat Syst Biol Unit, European Mol Biol Ctr, Barcelona, Spain

[2] Univ Pompeu Fabra, Ctr Genom Regulat, Inst Catalana Recerca & Estudis Avancats, Barcelona, Spain

来源：

NATURE METHODS | 2010年 / 7卷 / 09期

关键词：

TRANSFORMATION;

D O I：

10.1038/nmeth.1495

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Caenorhabditis elegans is an important model organism in biology, but until now no antibiotic selection markers have been successfully demonstrated for this species. We have developed a selection system using puromycin that allows the rapid and easy isolation of large populations of transgenic worms. This approach is sufficiently powerful to select single-copy transgenes, does not require any particular genetic background and also works in C. briggsae.

引用

页码：725 / U82

页数：5

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