Rapid selection of transgenic C. elegans using antibiotic resistance

被引:57
作者
Semple, Jennifer I. [1 ]
Garcia-Verdugo, Rosa [1 ]
Lehner, Ben [1 ,2 ]
机构
[1] UPF, Genom Regulat Syst Biol Unit, European Mol Biol Ctr, Barcelona, Spain
[2] Univ Pompeu Fabra, Ctr Genom Regulat, Inst Catalana Recerca & Estudis Avancats, Barcelona, Spain
关键词
TRANSFORMATION;
D O I
10.1038/nmeth.1495
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Caenorhabditis elegans is an important model organism in biology, but until now no antibiotic selection markers have been successfully demonstrated for this species. We have developed a selection system using puromycin that allows the rapid and easy isolation of large populations of transgenic worms. This approach is sufficiently powerful to select single-copy transgenes, does not require any particular genetic background and also works in C. briggsae.
引用
收藏
页码:725 / U82
页数:5
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