Ballistic transformation of Caenorhabditis elegans

被引:61
作者
Wilm, T
Demel, P
Koop, HU
Schnabel, H
Schnabel, R
机构
[1] Max Planck Inst Biochem, D-82152 Martinsried, Germany
[2] Univ Munich, Inst Bot, D-80638 Munich, Germany
关键词
gold particle; helium gun;
D O I
10.1016/S0378-1119(99)00043-8
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
A novel method to transform the nematode Caenorhabditis elegans is described. DNA coprecipitated with gold particles is shot at worms by means of a helium beam. Transformed worms are either identified by a dominant visible marker or selected by a conditional lethal system. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:31 / 35
页数:5
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