Enhancement of detection sensitivity for indirect photometric detection of anions and cations in capillary electrophoresis

被引：60

作者：

Johns, C ^{[1
]}

Macka, M ^{[1
]}

Haddad, PR ^{[1
]}

机构：

[1] Univ Tasmania, Sch Chem, Australian Ctr Res Separat Sci, Hobart, Tas 7001, Australia

来源：

ELECTROPHORESIS | 2003年 / 24卷 / 12-13期

关键词：

buffering; capillary electrophoresis; detector linearity; indirect photometric detection; review;

D O I：

10.1002/elps.200305446

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This review focuses on the indirect photometric detection of anions and cations by capillary electrophoresis. Special emphasis has been placed on the sensitivity of the technique and approaches taken to enhance detection limits. Theoretical considerations and requirements have been discussed, including buffering, detection sensitivity, separation of cations, and detector linearity. A series of tables detailing highly absorbing probes and the conditions of their use for indirect photometric detection are included.

引用

页码：2150 / 2167

页数：18

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