CAPILLARY ELECTROPHORESIS OF ORGANIC AND INORGANIC CATIONS WITH INDIRECT UV DETECTION

被引：190

作者：

BECK, W ^{[1
]}

ENGELHARDT, H ^{[1
]}

机构：

[1] UNIV SAARLAND,W-6600 SAARBRUCKEN,GERMANY

来源：

CHROMATOGRAPHIA | 1992年 / 33卷 / 7-8期

关键词：

CAPILLARY ELECTROPHORESIS; INDIRECT UV DETECTION; ALKALI METAL IONS; ALKALINE EARTH METAL IONS; ALIPHATIC AMINES;

D O I：

10.1007/BF02275908

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The determination of alkali and alkaline earth metal ions by capillary electrophoresis using indirect UV detection is described. With the identical system it is also possible to determine short chain aliphatic amines and alkanol amines within 4 minutes. Indirect UV detection is achieved at 214 nm with a background electrolyte containing 5 mmol L-1 imidazole. Linear calibration curves could be obtained for peak areas between 0.5 and 10 ppm. The detection limits are around 0.1 ppm (corresponding to 10 fmol in about 10 nL sample volume) for all cations and amines and 0.05 ppm for lithium. Practical applications demonstrate the applicability of this system in routine analysis.

引用

页码：313 / 316

页数：4

共 15 条

[1] CAPILLARY ZONE ELECTROPHORESIS OF RARE-EARTH-METALS WITH INDIRECT UV ABSORBENCY DETECTION [J].