Pathogenicity Determinants in Smut Fungi Revealed by Genome Comparison

被引：242

作者：

Schirawski, Jan ^{[1
,2
]}

Mannhaupt, Gertrud ^{[1
,3
]}

Muench, Karin ^{[1
]}

Brefort, Thomas ^{[1
]}

Schipper, Kerstin ^{[1
]}

Doehlemann, Gunther ^{[1
]}

Di Stasio, Maurizio ^{[1
]}

Roessel, Nicole ^{[1
]}

Mendoza-Mendoza, Artemio ^{[1
]}

Pester, Doris ^{[1
]}

Mueller, Olaf ^{[1
]}

Winterberg, Britta ^{[1
]}

Meyer, Elmar ^{[1
]}

Ghareeb, Hassan ^{[1
]}

Wollenberg, Theresa ^{[1
]}

Muensterkoetter, Martin ^{[3
]}

Wong, Philip ^{[3
]}

Walter, Mathias ^{[3
]}

Stukenbrock, Eva ^{[1
]}

Gueldener, Ulrich ^{[3
]}

Kahmann, Regine ^{[1
]}

机构：

[1] Max Planck Inst Terr Microbiol, Dept Organism Interact, D-35043 Marburg, Germany

[2] Univ Gottingen, Albrecht von Haller Inst Plant Sci, Dept Mol Biol Plant Microbe Interact, D-37073 Gottingen, Germany

[3] German Res Ctr Environm Hlth, Helmholtz Zentrum Munchen, Inst Bioinformat & Syst Biol, D-85764 Neuherberg, Germany

来源：

SCIENCE | 2010年 / 330卷 / 6010期

关键词：

USTILAGO-MAYDIS; SPORISORIUM; MAIZE;

D O I：

10.1126/science.1195330

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Biotrophic pathogens, such as the related maize pathogenic fungi Ustilago maydis and Sporisorium reilianum, establish an intimate relationship with their hosts by secreting protein effectors. Because secreted effectors interacting with plant proteins should rapidly evolve, we identified variable genomic regions by sequencing the genome of S. reilianum and comparing it with the U. maydis genome. We detected 43 regions of low sequence conservation in otherwise well-conserved syntenic genomes. These regions primarily encode secreted effectors and include previously identified virulence clusters. By deletion analysis in U. maydis, we demonstrate a role in virulence for four previously unknown diversity regions. This highlights the power of comparative genomics of closely related species for identification of virulence determinants.

引用

页码：1546 / 1548

页数：3