DNA supercoiling allows enhancer action over a large distance

被引：58

作者：

Liu, Y ^{[1
]}

Bondarenko, V ^{[1
]}

Ninfa, A ^{[1
]}

Studitsky, VM ^{[1
]}

机构：

[1] Wayne State Univ, Sch Med, Dept Biochem & Mol Biol, Detroit, MI 48201 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2001年 / 98卷 / 26期

关键词：

enhancers; Escherichia coli; transcription in vitro; NtrC activator; glnAp2; gene;

D O I：

10.1073/pnas.261477898

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Enhancers are regulatory DNA elements that can activate their genomic targets over a large distance. The mechanism of enhancer action over large distance is unknown. Activation of the glnAp2 promoter by NtrC-dependent enhancer in Escherichia coli was analyzed by using a purified system supporting multiple-round transcription in vitro. The data suggest that DNA supercoiling is an essential requirement for enhancer action over a large distance (2,500 bp) but not over a short distance (110 bp). DNA supercoiling facilitates functional enhancer-promoter communication over a large distance, probably by bringing the enhancer and promoter into close proximity.

引用

页码：14883 / 14888

页数：6

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