X-Ray Phase Nanotomography Resolves the 3D Human Bone Ultrastructure

被引：138

作者：

Langer, Max ^{[1
,2
]}

Pacureanu, Alexandra ^{[1
,2
,3
]}

Suhonen, Heikki ^{[2
]}

Grimal, Quentin ^{[4
]}

Cloetens, Peter ^{[2
]}

Peyrin, Francoise ^{[1
,2
]}

机构：

[1] Univ Lyon 1, CNRS, UMR5220, Inserm,U1044, F-69365 Lyon, France

[2] European Synchrotron Radiat Facil, F-38043 Grenoble, France

[3] Politeh Univ Bucharest, LAPI, Bucharest, Romania

[4] UPMC Univ Paris 06, CNRS, UMR7623, LIP, Paris, France

来源：

PLOS ONE | 2012年 / 7卷 / 08期

关键词：

SYNCHROTRON-RADIATION; TRABECULAR BONE; COMPACT-BONE; SECONDARY OSTEONS; CORTICAL BONE; CEMENT LINE; COLLAGEN; DENSITY; MINERALIZATION; ORIENTATION;

D O I：

10.1371/journal.pone.0035691

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Bone strength and failure are increasingly thought to be due to ultrastructural properties, such as the morphology of the lacuno-canalicular network, the collagen fiber orientation and the mineralization on the nanoscale. However, these properties have not been studied in 3D so far. Here we report the investigation of the human bone ultrastructure with X-ray phase nanotomography, which now provides the required sensitivity, spatial resolution and field of view. The 3D organization of the lacuno-canalicular network is studied in detail over several cells in osteonal and interstitial tissue. Nanoscale density variations are revealed and show that the cement line separating these tissues is hypermineralized. Finally, we show that the collagen fibers are organized as a twisted plywood structure in 3D.

引用

页数：7

共 37 条

[1]

Nanoscale X-Ray Microscopic Imaging of Mammalian Mineralized Tissue [J].