Differential roles of miR-199a-5p in radiation-induced autophagy in breast cancer cells

被引:125
作者
Yi, Heqing [1 ]
Liang, Bing [1 ]
Jia, Jie [2 ]
Liang, Nan [1 ]
Xu, Huiying [1 ]
Ju, Guizhi [1 ]
Ma, Shumei [1 ]
Liu, Xiaodong [1 ,3 ]
机构
[1] Jilin Univ, Sch Publ Hlth, Minist Hlth, Key Lab Radiobiol, Changchun 130021, Peoples R China
[2] Jilin Univ, China Japan Union Hosp, Changchun 130021, Peoples R China
[3] Jilin Univ, China Japan Union Hosp, Dept Radiol & Radiat Oncol, Changchun 130021, Peoples R China
基金
高等学校博士学科点专项科研基金;
关键词
MiR-199a-5p; DRAM1; Beclin1; Autophagy; Irradiation; GENE-EXPRESSION; UP-REGULATION; MICRORNA SIGNATURES; DOWN-REGULATION; TRANSLATION; RESISTANCE; TARGET; IDENTIFICATION; INHIBITION; MODULATION;
D O I
10.1016/j.febslet.2012.12.027
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
070307 [化学生物学]; 071010 [生物化学与分子生物学];
摘要
Autophagy is a self-degrading process that is triggered by diverse stimuli including ionizing radiation. In this study we show novel phenomena in which transfection of miR-199a-5p mimic significantly suppresses IR-induced autophagy in MCF7 cells, and up-regulates basal and IR-induced autophagy in MDA-MB-231 breast cancer cells. We also identify DRAM1 and Beclin1 as novel target genes for miR-199a-5p. Overexpression of miR-199a-5p inhibits DRAM1 and Beclin1 expression in MCF7 cells, while it enhances expression of these genes in MDA-MB-231 cells. Furthermore, we show that miR-199a-5p sensitizes MDA-MB-231 cells to irradiation. Therefore, our data identify miR-199a-5p as a novel and unique regulator of autophagy, which plays an important role in cancer biology and cancer therapy. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码:436 / 443
页数:8
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