P2Y isoforms operative in mouse taste cells

被引：26

作者：

Bystrova, MF ^{[1
]}

Yatzenko, YE ^{[1
]}

Fedorov, IV ^{[1
]}

Rogachevskaja, OA ^{[1
]}

Kolesnikov, SS ^{[1
]}

机构：

[1] Russian Acad Sci, Int Cell Biophys, Pushchino 142290, Moscow Region, Russia

来源：

CELL AND TISSUE RESEARCH | 2006年 / 323卷 / 03期

基金：

俄罗斯基础研究基金会;

关键词：

taste cells; P2Y receptors; intracellular Ca2+; reverse transcription/polymerase chain reaction; immunohistochemistry; mouse;

D O I：

10.1007/s00441-005-0098-8

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Recent functional evidence indicates that mouse taste cells express P2Y receptors coupled to IP3 production and Ca-2 mobilization. Our studies of the expression profile of particular P2Y isoforms in the taste tissue of the mouse have revealed that ATP and UTP equipotently mobilize intracellular Ca2+ at saturating concentrations, suggesting that common receptors for both nucleotides, i.e., P2Y(2) and P2Y(4) subtypes, might be involved. Reverse transcription/polymerase chain reaction and immunohistochemistry have confirmed the presence of P2Y(2) and P2Y(4) receptors in a population of taste bud cells from the circumvallate and foliate papillae. Transcripts for the P2Y(1) and P2Y(6) isoforms have also been detected in taste tissue preparations, this observation being consistent with the ADP and UDP responsiveness of taste cells. Together, our data suggest that P2Y(2) and P2Y(4) receptors play a predominant role in mediating taste cell responses to ATP and UTP.

引用

页码：377 / 382

页数：6

共 46 条

[1] Characterization of the UDP-glucose receptor (re-named here the P2Y14 receptor) adds diversity to the P2Y receptor family [J].

Abbracchio, MP ;

Boeynaems, JM ;

Barnard, EA ;

Boyer, JL ;

Kennedy, C ;

Miras-Portugal, MT ;

King, BF ;

Gachet, C ;

Jacobson, KA ;

Weisman, GA ;

Burnstock, G .

TRENDS IN PHARMACOLOGICAL SCIENCES, 2003, 24 (02) :52-55

[2] P2Y12 regulates platelet adhesion/activation, thrombus growth, and thrombus stability in injured arteries [J].

André, P ;

Delaney, SM ;

LaRocca, T ;

Vincent, D ;

DeGuzman, F ;

Jurek, M ;

Koller, B ;

Phillips, DR ;

Conley, PB .

JOURNAL OF CLINICAL INVESTIGATION, 2003, 112 (03) :398-406

[3]

Bailey M.A., 2004, NEPHRON PHYSIOL, V96, P79, DOI [DOI 10.1159/000076753, 10.1159/000076753]

[4] Calcium signaling mediated by P2Y receptors in mouse taste cells [J].

Baryshnikov, SG ;

Rogachevskaja, OA ;

Kolesnikov, SS .

JOURNAL OF NEUROPHYSIOLOGY, 2003, 90 (05) :3283-3294

[5] PPADS, an ATP antagonist, attenuates the effects of a moderately intense sound on cochlear mechanics [J].

Bobbin, RP .

HEARING RESEARCH, 2001, 156 (1-2) :10-16

[6] Molecular cloning and characterization of rat P2Y4 nucleotide receptor [J].

Bogdanov, YD ;

Wildman, SS ;

Clements, MP ;

King, BF ;

Burnstock, G .

BRITISH JOURNAL OF PHARMACOLOGY, 1998, 124 (03) :428-430

[7] Purinergic receptors on microglial cells:: functional expression in acute brain slices and modulation of microglial activation in vitro [J].

Boucsein, C ;

Zacharias, R ;

Färber, K ;

Pavlovic, S ;

Hanisch, UK ;

Kettenmann, H .

EUROPEAN JOURNAL OF NEUROSCIENCE, 2003, 17 (11) :2267-2276

[8] Introduction: P2 receptors [J].

Burnstock, G .

CURRENT TOPICS IN MEDICINAL CHEMISTRY, 2004, 4 (08) :793-803

[9] 3-DIMENSIONAL BRAIN METABOLIC IMAGING IN PATIENTS WITH TOXIC ENCEPHALOPATHY [J].

CALLENDER, TJ ;

MORROW, L ;

SUBRAMANIAN, K ;

DUHON, D ;

RISTOVV, M .

ENVIRONMENTAL RESEARCH, 1993, 60 (02) :295-319

[10] CHEMORECEPTORS OF CRUSTACEANS - SIMILARITIES TO RECEPTORS FOR NEUROACTIVE SUBSTANCES IN INTERNAL TISSUES [J].

CARR, WES ;

ACHE, BW ;

GLEESON, RA .

ENVIRONMENTAL HEALTH PERSPECTIVES, 1987, 71 :31-46

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