Label-free detection of DNA mutations by SPR: application to the early detection of inherited breast cancer

被引：64

作者：

Carrascosa, L. G. ^{[1
,2
]}

Calle, A. ^{[3
]}

Lechuga, L. M. ^{[1
,2
]}

机构：

[1] ETSE, CIN2 CSIC ICN, Res Ctr Nanosci & Nanotechnol, Nanobiosensors & Mol Nanobiophys Grp, Barcelona 08193, Spain

[2] CIBER BBN, Zaragoza 50018, Spain

[3] Inst Microelect, IMM CNM CSIC, Madrid 28760, Spain

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2009年 / 393卷 / 04期

关键词：

SPR biosensor; DNA hybridization; DNA mutation; BRCA-1; Breast cancer; SURFACE-PLASMON RESONANCE; BIOSENSOR TECHNOLOGY; OVARIAN-CANCER; NUCLEIC-ACIDS; HYBRIDIZATION; OLIGONUCLEOTIDE; GOLD; MONOLAYERS; EFFICIENCY; SEQUENCES;

D O I：

10.1007/s00216-008-2555-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A screening analysis of DNA hybridization and the presence of DNA mutations using an surface plasmon resonance (SPR) biosensor is shown. The influence of lateral and vertical spacers, as well as several hybridization conditions, was studied to optimize the differentiation between fully complementary and mismatched DNA strands. Our results demonstrated that SPR biosensors were able to detect mismatch sequences related to inherited breast cancer, with high specificity and sensitivity. Using PCR synthetic sequences as targets, mutant sequences were clearly discriminated from fully complementary ones, and detection limits below 50 nM were achieved.

引用

页码：1173 / 1182

页数：10

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