High-efficiency CAG-FLPe deleter mice in C57BL/6J background

被引：135

作者：

Kanki, H ^{[1
]}

Suzuki, H ^{[1
]}

Itohara, S ^{[1
]}

机构：

[1] RIKEN, Inst Phys & Chem Res, Lab Behav Genet, Brain Sci Inst, Wako, Saitama 3510198, Japan

来源：

EXPERIMENTAL ANIMALS | 2006年 / 55卷 / 02期

关键词：

CAG promoter; C57BL/6J; FLP(e); transgenic mouse;

D O I：

10.1538/expanim.55.137

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The increasing popularity of conditional knockout (KO) technology has resulted in the demand for efficient FLP deleter mice. In addition, FLP deleters are needed in genetic backgrounds that are suited to behavioral studies. We generated CAG-FLPe transgenic (Tg) mice with the C57BL/6J genetic background, which is one of the most commonly-used strains in behavioral studies. We assessed the recombination efficiency of the CAG-FLPe-Tg lines by crossing them with a mouse line carrying a FRT-PGK-neo-FRT cassette. Four of five independent CAG-FLPe lines induced recombination in most (91%-100%) of their progenies, although a small fraction (0%-30%, depending on the line) showed mosaic recombination patterns. These animals are highly potent as deleters of FRT cassettes and are useful for behavioral studies involving conditional KO mice.

引用

页码：137 / 141

页数：5

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