Identification of bloodmeals in haematophagous Diptera by cytochrome B heteroduplex analysis

被引:151
作者
Boakye, DA
Tang, J
Truc, P
Merriweather, A
Unnasch, TR
机构
[1] Univ Alabama, Div Geog Med, Birmingham, AL 35294 USA
[2] OCCGE, Inst Pierre Richet, Bouake, Cote Ivoire
关键词
Glossina; Simulium; cytochrome B; heteroduplex analysis; polymerase chain reaction; species identification; Ivory Coast;
D O I
10.1046/j.1365-2915.1999.00193.x
中图分类号
Q96 [昆虫学];
学科分类号
摘要
We developed a DNA assay for bloodmeal identification in haematophagous insects. Specific host cytochrome B gene sequences were amplified by PCR and classified on the basis of their mobility in a heteroduplex assay. In the blackfly Simulium damnosum s.l. (Diptera: Simuliidae), human cytochrome B DNA sequences were identifiable up to 3 days following ingestion of the bloodmeal. In the tsetse Glossina palpalis (Diptera: Glossinidae) collected from tsetse traps in Ivory Coast, bloodmeals were identified as taken from domestic pigs on the basis of their heteroduplex pattern and DNA sequence. Evidently the cytochrome B sequence shows sufficient interspecific variation to distinguish between mammalian host samples, while exhibiting minimal intraspecific variation. The stability of DNA in bloodmeals, for several days post-ingestion by haematophagous insects, allows PCR-HDA assays to be used reliably for host identification.
引用
收藏
页码:282 / 287
页数:6
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